High-sensitivity fluorescence anisotrophy detection of protein-folding events: Application to alpha-lactalbumin

High-sensitivity fluorescence anisotrophy detection of protein-folding events: Application to alpha-lactalbumin

An experimental procedure has been dervised to record simultaneously fluorescence intensity and fluroescence anisotropy. A photoelastic modulator on the excitation beam enables the anisotropy signal to be recorded in one pass using a single photomultiplier tube and eliminates the need for a polarize...

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Veröffentlicht in:Biophysical journal 2001-04, Vol.80 (4), p.1996
Hauptverfasser: Canet, Denis, Doering, Klaus, Dobson, Christopher M, Dupont, Yves
Format: Artikel
Sprache:eng
Schlagworte:
Fluorescence
Kinetics
Physics
Proteins
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Zusammenfassung:An experimental procedure has been dervised to record simultaneously fluorescence intensity and fluroescence anisotropy. A photoelastic modulator on the excitation beam enables the anisotropy signal to be recorded in one pass using a single photomultiplier tube and eliminates the need for a polarizer on the emission path.
ISSN:0006-3495
1542-0086