Effect of HSP27 on heat stress-induced oxidative stress injury and apoptosis in F98 cells

Objective To explore the effect of HSP27 on heat stress-induced oxidative stress injury and apoptosis in F98 cells. Methods The F98 cells were stress at 43 degrees for 60 minutes. After the stress, cells were collected at different time points (0, 3, 6, 9, 12h), the phosphorylation levels of HSP27 at various sites were examined by Western blotting. To explore the function of mitogen-activated protein kinase (MAPKs) pathway on HSP27 phosphorylation, MAPK inhibitors (SB203580, SP600125, PD98059) and adenoviruses expressing MAPK-activated protein kinase 2 (MK2) were employed. To decipher the mechanism of HSP27 phosphorylation on cell apoptosis, adenoviruses carrying HSP27 phosphorylation resistant mutations were transduced into the cell. Then, cell viability test kit, reactive oxygen species (ROS) test kit, apoptosis test kit, and caspase-3 activity test kit were used to detect the cell viability, ROS activities, and apoptosis of F98 cells. Results After heat stress, the expression of HSP27 increased slightly