Toxicity test of aluminium to Vibrio alginolyticus as a preliminary test of contaminated soil remediation

Aluminium pollution is currently becoming a serious issue in Jombang District, East Java, Indonesia. Aluminium contamination in soil was occurred due to the utilization of aluminium waste as roads and also river dams. This utilization of aluminium waste was very inappropriate and violate the regulation because its categorized as hazardous waste. One method that widely known to remediate metal contaminated soil is bioremediation. As a preliminary test, the toxicity of aluminium contaminated soil to bacteria need to be tested. Vibrio alginolyticus was known to have good ability as aluminium treatment agent. Toxicity test of aluminium contaminated soil to V. alginolyticus was conducted by using colony forming analysis. Several concentrations of AlCl3 i.e. 0, 50, 100, 200, 350, and 500 mg/L were used to test the bacterial growth in aluminium contaminated soil. Result indicated that V. alginolyticus still showing colony growth up to 350 mg/L equal to 262.17 mg/kg of aluminium contaminated soil. The concentration of 500 mg/L equal to 374.53 mg/kg completely inhibits the bacterial metabolism marked by the absence of V. alginolyticus growth.