In vivo evaluation of 2[variant prime]-deoxy-2[variant prime]-[18F]fluoro-5-iodo-1-[beta]-D-arabinofuranosyluracil ([18F]FIAU) and 2[variant prime]-deoxy-2[variant prime]-[18F]fluoro-5-ethyl-1-[beta]-D-arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression

In vivo evaluation of 2[variant prime]-deoxy-2[variant prime]-[18F]fluoro-5-iodo-1-[beta]-D-arabinofuranosyluracil ([18F]FIAU) and 2[variant prime]-deoxy-2[variant prime]-[18F]fluoro-5-ethyl-1-[beta]-D-arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression

Purpose FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. Methods In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. Results In vitr...

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Veröffentlicht in:European journal of nuclear medicine and molecular imaging 2007-06, Vol.34 (6), p.822
Hauptverfasser: Alauddin, Mian M, Shahinian, Antranic, Park, Ryan, Tohme, Michel, Fissekis, John D, Conti, Peter S
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Cancer
Gene expression
Nuclear medicine
Tomography
Tumors
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Zusammenfassung:Purpose FIAU and FEAU were evaluated in vitro and in vivo as markers for HSV1-tk gene expression. Methods In vitro and biodistribution studies were performed in wild type and transduced HT-29 cells using [14C]FIAU and [3H]FEAU. PET imaging was performed using [18F]FIAU and [18F]FEAU. Results In vitro uptake of [14C]FIAU in tk-positive cells was 39-fold, 49-fold, and 43-fold higher (p
ISSN:1619-7070
1619-7089
DOI:10.1007/s00259-006-0305-1