In vitro availability of zinc from infant foods with increasing phytic acid contents

An in vitro method was used to determine the availability of Zn from infant foods containing increasing amounts of phytate, and to quantify the effect of the phytate:Zn molar ratio on the availability. During the in vitro assay, digestive conditions of infants, younger and older than 4 months of age...

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Veröffentlicht in:	British journal of nutrition 2001-08, Vol.86 (2), p.241-247
Hauptverfasser:	Bosscher, Donwina, Lu, Zhengli, Janssens, Geert, Van Caillie-Bertrand, Micheline, Robberecht, Harry, Rycke, Herman De, De Wilde, Roland, Deelstra, Hendrik
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Analysis of Variance Animals Atomic absorption spectroscopy Babies Baby foods Bile Bioavailability Bioavailability of micronutrients Biological and medical sciences Biological Availability Cattle Chromatography, High Pressure Liquid Dialysis Dietary minerals Feeding. Feeding behavior Food Fundamental and applied biological sciences. Psychology Glycine max Hemodialysis Humans In vitro method Infant Infant Food - analysis Infant, Newborn Infants Liquid chromatography Micronutrients - analysis Milk Nutrition Phytate Phytic Acid - analysis Scientific imaging Soybeans Spectral analysis Spectrometry Spectrophotometry, Atomic Trace elements Vertebrates: anatomy and physiology, studies on body, several organs or systems Zinc Zinc - analysis
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Zusammenfassung:	An in vitro method was used to determine the availability of Zn from infant foods containing increasing amounts of phytate, and to quantify the effect of the phytate:Zn molar ratio on the availability. During the in vitro assay, digestive conditions of infants, younger and older than 4 months of age, were carefully simulated since the solubility of phytate–Zn complexes during digestion is pH dependent. Availability was measured with a continuous flow dialysis in vitro procedure with previous intralumen digestive stage. Zn concentrations were determined with flame atomic absorption spectrometry. Phytic acid content was measured with HPLC. Adding phytate to infant formula lowered Zn availability to 2·84 (SD 0·17) % WHEN THE PHYTATE:ZN MOLAR RATIO INCREASED TO 2·2 (P
ISSN:	0007-1145 1475-2662
DOI:	10.1079/BJN2001384