Mathematical Methods in the Calculation of the Zeta Potential of BSA

This article is focused on the zeta potential of bovine serum albumin (BSA). It is useful to know the zeta potentials of substances for further use of them, e.g. for adsorption or the stability of prepared solutions. We decided to create a database of zeta potential measurement. BSA was selected as a model protein for the measurements. The zeta potential was measured at different pH values, temperatures, concentrations and in the presence of KCl and CaCl 2 . All data were collected and used for the creation of the online application. The users of the online application can select an interval of zeta potentials required and the application shows the conditions under which the BSA solutions should be prepared. These measurements can be used to save time for further experiments, where the database can be used for the direct preparation of samples with desired properties instead of the lengthy preparation of the samples under different conditions and the selection of the appropriate parameters.