Agonists of PPAR-[alpha], PPAR-[gamma], and RXR inhibit the formation of foam cells from macrophages in mice with inflammation

We studied the effect of agonists of peroxisome proliferator-activated receptors α and γ and retinoid X receptors on the concentration and synthesis of lipids in macrophages of C57Bl/6 mice with inflammation induced by intraperitoneal injection of zymosan. We revealed a significant increase in [1- 14C]oleate incorporation into cholesterol esters and triglycerides, increase in the content of free cholesterol, cholesterol esters, and triglycerides, and formation of oil red-stained lipid inclusions in peritoneal macrophages 24 h after administration of zymosan in a dose of 50 mg/kg. Treatment with agonists of retinoid X receptors and peroxisome proliferator-activated receptors α and γ 30 min before and 12 h after zymosan injection decreased the synthesis of triglycerides and cholesterol esters, reduced the content of free cholesterol, cholesterol esters, and triglycerides in macrophages, and prevented the formation of cytoplasmic lipid inclusions in macrophage-derived foam cells during inflammation. [PUBLICATION ABSTRACT]