Inhibition effect of miR-598 on migration and invasion of non-small cell lung cancer by targeting MSI2

Objective To investigate the expression level of miR-598 in non-small cell lung cancer (NSCLC) tissues and cells lines, and determine its role on the migration and invasion of NSCLC cells. Methods The expression level of miR-598 in NSCLC tissues and cell lines was determined by real-time quantitative PCR (qRT-PCR). The miR-598 mimics or miR-598 inhibitors were transiently transfected into A549 cells to up- or down-regulate miR-598 expression, and then the effect of miR-598 on cell migration and invasion was detected by scratch test and Transwell chamber assay. The potential binding site of miR-598 on the MSI2 was predicted by using on-line bioinformation databases, and double luciferase assay was performed to verify the targeting regulatory relationship of miR-598 to MSI2. The migration and invasive ability of A549 cells was detected after transfected with MSI2 alone or with combined MSI2 and miR-598. Results The miR-598 expression was significantly down-regulated in NSCLC tissues compared to that in parac