Urine miRNAs expression profile in patients with Radioildine refractory

Background: Radioiodine refractory (RAI-R) is a long time process in which the thyroid lost the capability to uptake radioiodine for imaging and treatment of differentiated thyroid cancer (DTC). And with poor prognosis. Urine is the convenient and real non-invasive body fluid that containing molecules including proteins, RNA and metabolites, which reflect the conditions of the body. Methods: To unveil the expression profile of miRNA in urine for early biomarkers for RAI-R patients(n=12 female, age 51.2±15.7, from Shanghai 10th Peoples Hospital, from year of 2013-2015) , we collected RAI-R patients random urine and centrifugated, supernatant were enriched to nylon membrane, then miRNAs were extract by Trizol and reverse miRNA by tail adding(TAKARA product). Age and gender matched healthy control (n=10 mix, each mix containing 5 individual with equal amount of urine ) were selected from fitness examination volunteer. We measure the expression profile of 13 miRNAs that were documented to be up-regulated in DTC patients serum and four miRNAs including miR-16, miR-125a, miR-191 and miR-204 that to be reported to be the highest abundance in urine. Results: Using U6 as a internal control, we have found the ratio of delta Ct value (each miRNA Versus SNU6) of 13 up-regulated miRNAs up-regulated in DTC serum were no significant alterations. The expression of miR-16, miR-125a, miR-191 and miR-204, the miRNAs in urine were significantly up-regulated in patients with RAI-R. the p value of each above miRNA were p=0.0137 ,p=0.0007, p=0.0004 and p=0.0048 ,respectively, compared with normal control by nonparametric and uppaired t-test. Conclusions: Urine miRNAs expression profiles in RAI-R patients serve as an sensitive and promising biomarkers in assessing the course of RAI-R, and have a great potential to be applied in early diagnosis of RAI-R and monitor this process ahead of it to be occurred.