Identification and engineering of the key residues at the crevice-like binding site of lipases responsible for activity and substrate specificity

Objective Rational engineering of the crevice-like binding site of lipases for improvement of lipases’ catalytic properties. Resuts The residues located at the crevice-like binding site of four representative lipases including Thermomyces lanuginosus lipases (TLL and Lip), Rhizopus oryzae lipase (RO...

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