Quantitative characterization for dielectrophoretic behavior of biological cells using optical tweezers

We report a method to precisely quantify dielectrophoretic (DEP) forces and cutoff frequencies (fc) of viable and nonviable yeast cells. The method consists of a two-step process in which generated DEP forces act upon a cell through a micro-electrode device, followed by direct measurement of DEP for...

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Veröffentlicht in:Applied physics letters 2014-02, Vol.104 (5)
Hauptverfasser: Park, In Soo, Hee Park, Se, Woo Lee, Sang, Sung Yoon, Dae, Kim, Beop-Min
Format: Artikel
Sprache:eng
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Zusammenfassung:We report a method to precisely quantify dielectrophoretic (DEP) forces and cutoff frequencies (fc) of viable and nonviable yeast cells. The method consists of a two-step process in which generated DEP forces act upon a cell through a micro-electrode device, followed by direct measurement of DEP forces using optical tweezers. DEP behaviors of viable and nonviable yeast cells are monitored as a function of AC frequency. We believe that the proposed method can be used as a powerful platform for cell-based assays to characterize the DEP behavior of various cell types including cancer and normal cells.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.4862746