Optimization of simultaneous enzymatic inactivation and extraction of linamarin from cassava leaf by UV-assisted photobioextraction

This research objective was to optimize the linamarin production of cassava leaf through simultaneous linamarase inactivation and osmotic dehydration by applied UV-photobioextractor technology. The optimization of the linamarin production process parameters was conducted by applied Response Surface Methodology (RSM) methods. The fixed parameters were comprised of speed rotary mixer of 75 rpm, chopper blades rotating speed of 125 rpm, drying agent of magnesium sulfate, and dryer temperature of 80°C. Meanwhile the process variables were comprised of ratio of solvent-cassava leaves (10: 1; 15: 1; 20: 1; 25: 1% (w/w)), the concentration of ethanol (80; 85; 90; 95%), and the concentration of drying agent (5; 7.5; 10; 12.5% (w/w)). The optimization of the linamarin production by applied RSM showed that the concentration of solvent (L) was the most influencing parameter process, meanwhile the ratio of solvent-cassava leaves and the concentration of drying agent had no significant effect to levels of linamarin extraction. The research also showed that along with the increasing of concentration of solvents and solvent-feed ratio, the linamarin yield extract was also increasing. The highest linamarin yield was obtained from the simultaneous linamarase inactivation and osmotic dehydration in 90% of ethanol concentration and solvent feed ratio of 15: 1.