Revisiting the Chrome Azurol S Assay for Various Metal Ions

Siderophores play an important role in the solubilisation and mobilization of iron (III) and various metal ions. To have a useful method to test siderophores in culture supernatants for their metal binding affinity, we redesigned and optimized the liquid CAS-assay for selected metal ions. CAS-assay...

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Veröffentlicht in:	Solid state phenomena 2017-08, Vol.262, p.509-512
Hauptverfasser:	Schwabe, Rïngo, Vater, Sabine, Mehnert, Marika, Retamal-Morales, Gerardo, Tischler, Dirk, Levicán, Gloria J., Schlömann, Michael, Heine, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum Assaying Chelates Copper Culture Ions Iron Metal ions Quantitative analysis Test procedures
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Siderophores play an important role in the solubilisation and mobilization of iron (III) and various metal ions. To have a useful method to test siderophores in culture supernatants for their metal binding affinity, we redesigned and optimized the liquid CAS-assay for selected metal ions. CAS-assay solutions were calibrated with desferrioxamine B in different concentrations to calculate DFOB-equivalents to get a semi-quantitative evaluation. With these assay solutions, we were able to test siderophores in culture supernatants for their ability to chelate with Fe, Al, Ga, Cu, V and As.
ISSN:	1012-0394 1662-9779 1662-9779
DOI:	10.4028/www.scientific.net/SSP.262.509