GALR1/GALR2 Knockdown rats block the Depression and Anxiogenic effects induced by GAL(1-15): The Heterodimer GALR1/GALR2 as a target of GAL(1-15)

The Galanin N-terminal fragment(1-15) [GAL(1-15)] induces depressant-and anxiogenic-like actions. In this work, we have studied the role of GALR2 and GALR1 on the effects of GAL(1-15) in the Forced Swimming Test (FST) and Open Field Test (OFT) using siRNA GALR2 and GALR1 knockdown rats. Rats were injected with GAL(l-15)3nmol, GALR2antagonist M871 3nmol in combination or alone 15 before the test. The time of immobility.climbing and swimming were recorded in FST and Time and entries in the center in the OFT. In other experiment, rats were injected Intracerebroventricular (icv) with siRNA-GALR2 or siRNA-GALRl to generate the GALRknockdown rats. These knockdown rats were used in the OFT/FST after receiving icv GAL(l-15)3nmol 15min before the test Vehicle was used as control. In the FST, M871 significantly blocked the increased immobility (p