Immobilization of Laccase from T. versicolor on Nanofiber Matrix

Immobilization of Laccase from T. versicolor on Nanofiber Matrix

Prepared nanofiber matrices based on polyamide 6 and polyamide 6/chitosan with numerous amine groups were tested on enzyme immobilization. Laccase from Trametes versicolor was immobilized on both nanofiber sheets either via glutaraldehyde and hexamethylenediamine activation, or via adsorption follow...

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Veröffentlicht in:Materials science forum 2018-10, Vol.937, p.123-128
Hauptverfasser: Maryška, Jiří, Vaňátková, Petra, Schaabová, Markéta, Maryšková, Milena
Format: Artikel
Sprache:eng
Schlagworte:
Laccase
Nanofibers
Silicon
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Zusammenfassung:Prepared nanofiber matrices based on polyamide 6 and polyamide 6/chitosan with numerous amine groups were tested on enzyme immobilization. Laccase from Trametes versicolor was immobilized on both nanofiber sheets either via glutaraldehyde and hexamethylenediamine activation, or via adsorption followed by glutaraldehyde crosslinking. Both types of the attachment were successful, however, the adsorption method provided immobilized laccase with enhanced operational stability.
ISSN:0255-5476
1662-9752
1662-9752
DOI:10.4028/www.scientific.net/MSF.937.123