In Vitro Evidence for an Intracellular Site of Angiotensin Action

In Vitro Evidence for an Intracellular Site of Angiotensin Action

To differentiate the relative effects of nuclear and cell surface angiotensin II (Ang II) receptors, we mutated the angiotensinogen cDNA by removing the signal sequence-encoding region to produce a nonsecreted form of angiotensinogen [Ang(−S)Exp]. Rat hepatoma cells (which produce renin and angioten...

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Veröffentlicht in:Circulation research 2001-12, Vol.89 (12), p.1138-1146
Hauptverfasser: Cook, Julia L, Zhang, Zhuo, Re, Richard N
Format: Artikel
Sprache:eng
Schlagworte:
Alternative Splicing
Angiotensin Receptor Antagonists
Angiotensinogen - genetics
Angiotensinogen - metabolism
Angiotensins - metabolism
Angiotensins - pharmacology
Animals
Antihypertensive Agents - pharmacology
Benzimidazoles - pharmacology
Biological and medical sciences
Bromodeoxyuridine
Carcinoma, Hepatocellular - metabolism
Cell Division - drug effects
Cell Division - genetics
Clone Cells - metabolism
Fundamental and applied biological sciences. Psychology
Intracellular Fluid - metabolism
Liver. Bile. Biliary tracts
Losartan - pharmacology
Mitotic Index
Mutagenesis, Site-Directed
Oligonucleotides, Antisense - pharmacology
Peptidyl-Dipeptidase A - biosynthesis
Polymerase Chain Reaction
Rats
Receptors, Angiotensin - metabolism
Receptors, Cytoplasmic and Nuclear - antagonists & inhibitors
Receptors, Cytoplasmic and Nuclear - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Renin - antagonists & inhibitors
Renin - biosynthesis
Renin - genetics
Sequence Deletion
Tetrazoles - pharmacology
Transfection
Tumor Cells, Cultured
Vertebrates: digestive system
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Zusammenfassung:To differentiate the relative effects of nuclear and cell surface angiotensin II (Ang II) receptors, we mutated the angiotensinogen cDNA by removing the signal sequence-encoding region to produce a nonsecreted form of angiotensinogen [Ang(−S)Exp]. Rat hepatoma cells (which produce renin and angiotensin-converting enzyme mRNAs) were stably transfected with Ang(−S)Exp/pSVL (or a corresponding control) expression plasmid, and mitotic indices were measured for stably transfected cell lines. Experimental clonal cell lines demonstrate an average of 33±4.4% (P
ISSN:0009-7330
1524-4571
DOI:10.1161/hh2401.101270