Acid Prohormone Sequence Determines Size, Shape, and Docking of Secretory Vesicles in Atrial Myocytes

ABSTRACT—How vesicles are born in the trans-Golgi network and reach their docking sites at the plasma membrane is still largely unknown and is investigated in the present study on live, primary cultured atrial cardiomyocytes. Secretory vesicles (n=422) are visualized by expressing fusion proteins of...

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Veröffentlicht in:	Circulation research 2001-08, Vol.89 (3), p.e23-e29
Hauptverfasser:	Baertschi, Alex J, Monnier, Dominique, Schmidt, Uta, Levitan, Edwin S, Fakan, Stanislas, Roatti, Angela
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Newborn Atrial Natriuretic Factor - genetics Binding Sites - physiology Biological Transport - physiology Calcium - metabolism Cell Membrane - metabolism Cell Membrane - ultrastructure Cells, Cultured Green Fluorescent Proteins Heart Atria - metabolism Heart Atria - ultrastructure Heart Ventricles - cytology Heart Ventricles - metabolism Luminescent Proteins - genetics Mice Microscopy, Immunoelectron Microspheres Mutagenesis, Site-Directed Myocardium - metabolism Myocardium - ultrastructure Particle Size Protein Precursors - genetics Protein Sorting Signals - physiology Rats Rats, Sprague-Dawley Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Secretory Vesicles - metabolism Secretory Vesicles - ultrastructure Signal Transduction - physiology Structure-Activity Relationship trans-Golgi Network - metabolism
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Zusammenfassung:	ABSTRACT—How vesicles are born in the trans-Golgi network and reach their docking sites at the plasma membrane is still largely unknown and is investigated in the present study on live, primary cultured atrial cardiomyocytes. Secretory vesicles (n=422) are visualized by expressing fusion proteins of proatrial natriuretic peptide (proANP) and green fluorescent protein. Myocytes expressing fusion proteins with intact proANP display two populations of fluorescent vesicles with apparent diameters of 120 and 175 nm, moving at a top velocity of 0.3 μm/s. The number of docked vesicles is significantly correlated with the number of mobile vesicles (r =0.71, P
ISSN:	0009-7330 1524-4571
DOI:	10.1161/hh1501.095715