In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells
Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrason...
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| Veröffentlicht in: | Physiological research 2007, Vol.56 Suppl 1, p.S27-S32 |
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| creator | Kolárová, H Bajgar, R Tománková, K Krestýn, E Dolezal, L Hálek, J |
| description | Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment. |
| doi_str_mv | 10.33549/physiolres.931298 |
| format | Article |
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There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.931298</identifier><identifier>PMID: 17552898</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Adenocarcinoma - therapy ; Apoptosis ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Breast Neoplasms - therapy ; Cavitation ; Cell Line, Tumor ; Cell Shape - drug effects ; Cells ; Dose-Response Relationship, Drug ; Drugs ; Female ; Fluorescence ; Free radicals ; Humans ; Light emitting diodes ; Organometallic Compounds - pharmacology ; Organometallic Compounds - therapeutic use ; Photochemotherapy ; Photodynamic therapy ; Photosensitizing Agents - pharmacology ; Photosensitizing Agents - therapeutic use ; Reactive Oxygen Species - metabolism ; Regression analysis ; Studies ; Time Factors ; Ultrasonic Therapy</subject><ispartof>Physiological research, 2007, Vol.56 Suppl 1, p.S27-S32</ispartof><rights>Copyright Institute of Physiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-a37249fc1670d627fff3b0b3a154b38c1315941ac374aa8759d8a1a366f06d4b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17552898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kolárová, H</creatorcontrib><creatorcontrib>Bajgar, R</creatorcontrib><creatorcontrib>Tománková, K</creatorcontrib><creatorcontrib>Krestýn, E</creatorcontrib><creatorcontrib>Dolezal, L</creatorcontrib><creatorcontrib>Hálek, J</creatorcontrib><title>In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment.</description><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Adenocarcinoma - therapy</subject><subject>Apoptosis</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - therapy</subject><subject>Cavitation</subject><subject>Cell Line, Tumor</subject><subject>Cell Shape - drug effects</subject><subject>Cells</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drugs</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Free radicals</subject><subject>Humans</subject><subject>Light emitting diodes</subject><subject>Organometallic Compounds - pharmacology</subject><subject>Organometallic Compounds - therapeutic use</subject><subject>Photochemotherapy</subject><subject>Photodynamic therapy</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Regression analysis</subject><subject>Studies</subject><subject>Time Factors</subject><subject>Ultrasonic Therapy</subject><issn>0862-8408</issn><issn>1802-9973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpFkEtLAzEQx4Motla_gAcJ3rfmsY_kKMVHoeBFz0s2ybYp22TNo7jf3mALXmYO85v_DD8A7jFaUlqV_GncTcG4weuw5BQTzi7AHDNECs4begnmiNWkYCViM3ATwh4h0qCGXoMZbqqKMM7m4Li28GiidzDEpCboeui1kNEcNXQ_01ZbGEYtjQ5w9E6lPHEWquSN3cJx56JTkxUHI2HcaS_GCRoL0xC9CC5ZVYxexxwYtYJSWKk9lHoYwi246sUQ9N25L8DX68vn6r3YfLytV8-bQtKGxELkWvJe4rpBqiZN3_e0Qx0VuCo7yiSmuOIlFpkuhWBNxRUTWNC67lGtMrIAj6fc_Px30iG2e5e8zSdbggkpESlxhsgJkt6F4HXfjt4chJ9ajNo_0-2_6fZkOi89nJNTd9Dqf-Wslv4Cl21_og</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Kolárová, H</creator><creator>Bajgar, R</creator><creator>Tománková, K</creator><creator>Krestýn, E</creator><creator>Dolezal, L</creator><creator>Hálek, J</creator><general>Institute of 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vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells</title><author>Kolárová, H ; Bajgar, R ; Tománková, K ; Krestýn, E ; Dolezal, L ; Hálek, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-a37249fc1670d627fff3b0b3a154b38c1315941ac374aa8759d8a1a366f06d4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Adenocarcinoma - therapy</topic><topic>Apoptosis</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Breast Neoplasms - therapy</topic><topic>Cavitation</topic><topic>Cell Line, Tumor</topic><topic>Cell Shape - drug effects</topic><topic>Cells</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drugs</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Free radicals</topic><topic>Humans</topic><topic>Light emitting diodes</topic><topic>Organometallic Compounds - pharmacology</topic><topic>Organometallic Compounds - therapeutic use</topic><topic>Photochemotherapy</topic><topic>Photodynamic therapy</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Photosensitizing Agents - therapeutic use</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Regression analysis</topic><topic>Studies</topic><topic>Time Factors</topic><topic>Ultrasonic Therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kolárová, H</creatorcontrib><creatorcontrib>Bajgar, R</creatorcontrib><creatorcontrib>Tománková, K</creatorcontrib><creatorcontrib>Krestýn, E</creatorcontrib><creatorcontrib>Dolezal, L</creatorcontrib><creatorcontrib>Hálek, 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research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kolárová, H</au><au>Bajgar, R</au><au>Tománková, K</au><au>Krestýn, E</au><au>Dolezal, L</au><au>Hálek, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells</atitle><jtitle>Physiological research</jtitle><addtitle>Physiol Res</addtitle><date>2007</date><risdate>2007</risdate><volume>56 Suppl 1</volume><spage>S27</spage><epage>S32</epage><pages>S27-S32</pages><issn>0862-8408</issn><eissn>1802-9973</eissn><abstract>Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>17552898</pmid><doi>10.33549/physiolres.931298</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenocarcinoma - metabolism Adenocarcinoma - pathology Adenocarcinoma - therapy Apoptosis Breast Neoplasms - metabolism Breast Neoplasms - pathology Breast Neoplasms - therapy Cavitation Cell Line, Tumor Cell Shape - drug effects Cells Dose-Response Relationship, Drug Drugs Female Fluorescence Free radicals Humans Light emitting diodes Organometallic Compounds - pharmacology Organometallic Compounds - therapeutic use Photochemotherapy Photodynamic therapy Photosensitizing Agents - pharmacology Photosensitizing Agents - therapeutic use Reactive Oxygen Species - metabolism Regression analysis Studies Time Factors Ultrasonic Therapy |
| title | In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells |
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