Spectrophotometric Determination of Trace Amount of some Aromatic Amines in Pharmaceutical Formulations, Wastewater and Biological Fluids

A simple, accurate, sensitive and rapid method for the determination of some aromatic amines in either the pure form or in pharmaceutical formulation or in biological fluids or in waste water sample is proposed. The method is based on the formation of colored compounds with sodium 1,2-naphthoquinone-4-sulphonate and cetyltrimethyl ammonium bromide in alkaline medium. Beer’s law of colored species is obeyed in the range of concentration of 0.5 – 24 µg ml-1. The molar absorptivity was found to be in the range of 3.7504x103 – 1.1180x104 l mol-1 cm-1. Results obtained by using the proposed method for various pharmaceutical preparations compare favorably with those obtained by the official method.