Observation of DNA and protein distributions in mammalian cell nuclei using STXM

Observation of DNA and protein distributions in mammalian cell nuclei using STXM

A whole A549 cell and isolated nuclei of HeLa S3 cells in the apoptotic process were investigated by using a scanning transmission X-ray microscope (STXM) in the UVSOR Synchrotron (Okazaki, Japan). Near edge X-ray absorption fine structures (NEXAFS) of DNA and histone in the N K-edge region were mea...

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Hauptverfasser: Ohigashi, Takuji, Ito, Atsushi, Shinohara, Kunio, Tone, Shigenobu, Kado, Masataka, Inagaki, Yuichi, Wang, Yu-Fu, Kosugi, Nobuhiro
Format: Tagungsbericht
Sprache:eng
Schlagworte:
Apoptosis
Deoxyribonucleic acid
DNA
Nuclei (cytology)
Proteins
X ray absorption
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Zusammenfassung:A whole A549 cell and isolated nuclei of HeLa S3 cells in the apoptotic process were investigated by using a scanning transmission X-ray microscope (STXM) in the UVSOR Synchrotron (Okazaki, Japan). Near edge X-ray absorption fine structures (NEXAFS) of DNA and histone in the N K-edge region were measured as reference and their distribution in the nuclei was determined by using these reference spectra. The four stages of the apoptosis were successfully distinguished.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.4937521