Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7
Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present stu...
Gespeichert in:
Veröffentlicht in: | Oriental journal of chemistry 2016-01, Vol.32 (6), p.3099-3111 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 3111 |
---|---|
container_issue | 6 |
container_start_page | 3099 |
container_title | Oriental journal of chemistry |
container_volume | 32 |
creator | Keykanlu, Hadis Zibaei, Saeid Ardjmand, Mehdi Safekordi, Ali |
description | Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML) and Lactoferrin (LF) of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB) nanoparticles, were examined on MCF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD) spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm), but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF) to +21.61(LF 110.66µg/ml) and +20.93(ML 0.1µg/ml) and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM), the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data. |
doi_str_mv | 10.13005/ojc/320631 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2121683588</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2121683588</sourcerecordid><originalsourceid>FETCH-LOGICAL-c298t-560c82dae1ed83f41f2392a7976682d12573cc8301eb174e6ce2e5f5869fd8d13</originalsourceid><addsrcrecordid>eNpVUltv0zAUDggkqrEn_oAlXoYgzJfm9thmLUPqtggKQrxEnnOsukrscOxIlF-P15YH_GL583c70kmSN4x-ZILS7Nrt1bXgNBfseTLjXLA0o6J6kcxoVdCUcvrjVXLp_Z7GU81FzrLZs80X6J3sSDOh0QY6cge9CcFYIm1HNlIFpwExvp0lDaDuJ4fOqXDoyRLdYDog99K6UWIwqgdPrpr1wzK9H_27o8XqtxyMBRJ2QG6MD2gep2CimdOkOYvIV_MHPpCfECRpXAAbjOyP6tpZbXCQ_xTbHRgkC6XA-yNkj8b_N_huJNniYQxu3ElL1k-VwSuwCk6mBtXUS4x91A6d8QO5qm9OdU3wZBHz01pGOpKV1qAiFoNupyG6LRGkD-T8XUPfk83TfHf1unidvNSy93B5vi-Sb-vVtr5NNw-fPteLTap4VYY0y6kqeSeBQVcKPWeai4rLoiryPOKMZ4VQqhSUwSMr5pAr4JDprMwr3ZUdExfJ25PviO7XBD60ezehjZEtZ5zlpcjKMrLen1gKnfcIuh3RDBIPLaPtcWPauDHtaWPEX2XNt_o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2121683588</pqid></control><display><type>article</type><title>Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Keykanlu, Hadis ; Zibaei, Saeid ; Ardjmand, Mehdi ; Safekordi, Ali</creator><creatorcontrib>Keykanlu, Hadis ; Zibaei, Saeid ; Ardjmand, Mehdi ; Safekordi, Ali</creatorcontrib><description>Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML) and Lactoferrin (LF) of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB) nanoparticles, were examined on MCF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD) spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm), but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF) to +21.61(LF 110.66µg/ml) and +20.93(ML 0.1µg/ml) and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM), the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data.</description><identifier>ISSN: 0970-020X</identifier><identifier>EISSN: 2231-5039</identifier><identifier>DOI: 10.13005/ojc/320631</identifier><language>eng</language><publisher>Bhopal: Oriental Scientific Publishing Company</publisher><subject>Anticancer properties ; Apoptosis ; Breast cancer ; Cancer ; Cancer therapies ; Cations ; Cell death ; Chemotherapy ; Chromatography ; Dichroism ; Drug delivery systems ; Fluorescence ; Health care ; Kinases ; Molecular weight ; Morphology ; Nanoemulsions ; Nanoparticles ; Particle size ; Particle size distribution ; Peptides ; Perfluorocarbons ; Physical chemistry ; Proteins ; Scanning electron microscopy ; Sodium ; Tryptophan ; Ultrasonic imaging ; Zeta potential</subject><ispartof>Oriental journal of chemistry, 2016-01, Vol.32 (6), p.3099-3111</ispartof><rights>2016. This work is published under http://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c298t-560c82dae1ed83f41f2392a7976682d12573cc8301eb174e6ce2e5f5869fd8d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Keykanlu, Hadis</creatorcontrib><creatorcontrib>Zibaei, Saeid</creatorcontrib><creatorcontrib>Ardjmand, Mehdi</creatorcontrib><creatorcontrib>Safekordi, Ali</creatorcontrib><title>Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7</title><title>Oriental journal of chemistry</title><description>Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML) and Lactoferrin (LF) of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB) nanoparticles, were examined on MCF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD) spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm), but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF) to +21.61(LF 110.66µg/ml) and +20.93(ML 0.1µg/ml) and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM), the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data.</description><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Cations</subject><subject>Cell death</subject><subject>Chemotherapy</subject><subject>Chromatography</subject><subject>Dichroism</subject><subject>Drug delivery systems</subject><subject>Fluorescence</subject><subject>Health care</subject><subject>Kinases</subject><subject>Molecular weight</subject><subject>Morphology</subject><subject>Nanoemulsions</subject><subject>Nanoparticles</subject><subject>Particle size</subject><subject>Particle size distribution</subject><subject>Peptides</subject><subject>Perfluorocarbons</subject><subject>Physical chemistry</subject><subject>Proteins</subject><subject>Scanning electron microscopy</subject><subject>Sodium</subject><subject>Tryptophan</subject><subject>Ultrasonic imaging</subject><subject>Zeta potential</subject><issn>0970-020X</issn><issn>2231-5039</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpVUltv0zAUDggkqrEn_oAlXoYgzJfm9thmLUPqtggKQrxEnnOsukrscOxIlF-P15YH_GL583c70kmSN4x-ZILS7Nrt1bXgNBfseTLjXLA0o6J6kcxoVdCUcvrjVXLp_Z7GU81FzrLZs80X6J3sSDOh0QY6cge9CcFYIm1HNlIFpwExvp0lDaDuJ4fOqXDoyRLdYDog99K6UWIwqgdPrpr1wzK9H_27o8XqtxyMBRJ2QG6MD2gep2CimdOkOYvIV_MHPpCfECRpXAAbjOyP6tpZbXCQ_xTbHRgkC6XA-yNkj8b_N_huJNniYQxu3ElL1k-VwSuwCk6mBtXUS4x91A6d8QO5qm9OdU3wZBHz01pGOpKV1qAiFoNupyG6LRGkD-T8XUPfk83TfHf1unidvNSy93B5vi-Sb-vVtr5NNw-fPteLTap4VYY0y6kqeSeBQVcKPWeai4rLoiryPOKMZ4VQqhSUwSMr5pAr4JDprMwr3ZUdExfJ25PviO7XBD60ezehjZEtZ5zlpcjKMrLen1gKnfcIuh3RDBIPLaPtcWPauDHtaWPEX2XNt_o</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Keykanlu, Hadis</creator><creator>Zibaei, Saeid</creator><creator>Ardjmand, Mehdi</creator><creator>Safekordi, Ali</creator><general>Oriental Scientific Publishing Company</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20160101</creationdate><title>Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7</title><author>Keykanlu, Hadis ; Zibaei, Saeid ; Ardjmand, Mehdi ; Safekordi, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-560c82dae1ed83f41f2392a7976682d12573cc8301eb174e6ce2e5f5869fd8d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Cations</topic><topic>Cell death</topic><topic>Chemotherapy</topic><topic>Chromatography</topic><topic>Dichroism</topic><topic>Drug delivery systems</topic><topic>Fluorescence</topic><topic>Health care</topic><topic>Kinases</topic><topic>Molecular weight</topic><topic>Morphology</topic><topic>Nanoemulsions</topic><topic>Nanoparticles</topic><topic>Particle size</topic><topic>Particle size distribution</topic><topic>Peptides</topic><topic>Perfluorocarbons</topic><topic>Physical chemistry</topic><topic>Proteins</topic><topic>Scanning electron microscopy</topic><topic>Sodium</topic><topic>Tryptophan</topic><topic>Ultrasonic imaging</topic><topic>Zeta potential</topic><toplevel>online_resources</toplevel><creatorcontrib>Keykanlu, Hadis</creatorcontrib><creatorcontrib>Zibaei, Saeid</creatorcontrib><creatorcontrib>Ardjmand, Mehdi</creatorcontrib><creatorcontrib>Safekordi, Ali</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Oriental journal of chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keykanlu, Hadis</au><au>Zibaei, Saeid</au><au>Ardjmand, Mehdi</au><au>Safekordi, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7</atitle><jtitle>Oriental journal of chemistry</jtitle><date>2016-01-01</date><risdate>2016</risdate><volume>32</volume><issue>6</issue><spage>3099</spage><epage>3111</epage><pages>3099-3111</pages><issn>0970-020X</issn><eissn>2231-5039</eissn><abstract>Acording to the prevalence of cancer in today's societies, it is too important to find new drugs for that treatment. Cationic antimicrobial peptides that are able to eliminate a wide range of bacteria, fungi, parasites, viruses and unicellular, are natural anti-cancer agents. In the present study, the effect of cationic peptides of bee venom Melittin (ML) and Lactoferrin (LF) of camel milk which was loaded on the Perfluorooctyl Bromide (PFOB) nanoparticles, were examined on MCF7 cells. Nanoparticles using oil in water emulsion was prepared by ultra-thoracic and ultrasonic. Then ML and LF were separately added to the nanoparticles then incorporation of them were confirmed by tryptophan fluorescence and circular dichroism (CD) spectra. The mean diameter of the nanoparticles did not change after addition of ML and LF (~100 nm), but as expected, the zeta potential of the nanoparticles with increasing of ML and LF concentrations to the preformed nanoparticles increased from -18.43(without ML and LF) to +21.61(LF 110.66µg/ml) and +20.93(ML 0.1µg/ml) and +23.75(LF 110.66µg/ml and ML 0.1µg/ml. By using of scanning electron microscopy (SEM), the morphology and structure of nanoparticles was investigated, homogeneity and uniformity during processing and loading of ML and LF was observed. Effectiveness of nanoparticles loaded on cancer cells was performed with MTT test. The results showed that ML and LF had a positive effect on cancer cell death. Percent of cell death was higher when ML and LF was loaded on nanoparticles. With increase of ML and LF concentration loading on nanoparticles, increases cancer cell death. Minitab software was used to analyze some data.</abstract><cop>Bhopal</cop><pub>Oriental Scientific Publishing Company</pub><doi>10.13005/ojc/320631</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0970-020X |
ispartof | Oriental journal of chemistry, 2016-01, Vol.32 (6), p.3099-3111 |
issn | 0970-020X 2231-5039 |
language | eng |
recordid | cdi_proquest_journals_2121683588 |
source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
subjects | Anticancer properties Apoptosis Breast cancer Cancer Cancer therapies Cations Cell death Chemotherapy Chromatography Dichroism Drug delivery systems Fluorescence Health care Kinases Molecular weight Morphology Nanoemulsions Nanoparticles Particle size Particle size distribution Peptides Perfluorocarbons Physical chemistry Proteins Scanning electron microscopy Sodium Tryptophan Ultrasonic imaging Zeta potential |
title | Reload Purified Melittin and Lactoferrin on Perfluorooctyl Bromide Nanoparticles (PFOB-Nps) and Examine the Distribution of Particle Size, Zeta Potential and Confirmation of Their Accession on the Nanoparticles Via Tryptophan Fluorescence and Circular Dichroism (CD) and its Anti-Cancer Effects on Human Breast Cancer Cell Line MCF7 |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T01%3A21%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reload%20Purified%20Melittin%20and%20Lactoferrin%20on%20Perfluorooctyl%20Bromide%20Nanoparticles%20(PFOB-Nps)%20and%20Examine%20the%20Distribution%20of%20Particle%20Size,%20Zeta%20Potential%20and%20Confirmation%20of%20Their%20Accession%20on%20the%20Nanoparticles%20Via%20Tryptophan%20Fluorescence%20and%20Circular%20Dichroism%20(CD)%20and%20its%20Anti-Cancer%20Effects%20on%20Human%20Breast%20Cancer%20Cell%20Line%20MCF7&rft.jtitle=Oriental%20journal%20of%20chemistry&rft.au=Keykanlu,%20Hadis&rft.date=2016-01-01&rft.volume=32&rft.issue=6&rft.spage=3099&rft.epage=3111&rft.pages=3099-3111&rft.issn=0970-020X&rft.eissn=2231-5039&rft_id=info:doi/10.13005/ojc/320631&rft_dat=%3Cproquest_cross%3E2121683588%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2121683588&rft_id=info:pmid/&rfr_iscdi=true |