Analysis of DAPI and SYBR Green I as Alternatives to Ethidium Bromide for Nucleic Acid Staining in Agarose Gel Electrophoresis

DNA electrophoresis and staining is a common procedure in biochemistry laboratories, but the use of ethidium bromide (EB) for DNA detection is worrisome as EB is a mutagen and probable carcinogen. Five alternative stains were evaluated for DNA detection, safety, cost, and ease of use: BlueView, meth...

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Veröffentlicht in:Journal of chemical education 2003-11, Vol.80 (11), p.1292
Hauptverfasser: Bourzac, Kevin M, LaVine, Lori J, Rice, Margaret S
Format: Artikel
Sprache:eng
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Zusammenfassung:DNA electrophoresis and staining is a common procedure in biochemistry laboratories, but the use of ethidium bromide (EB) for DNA detection is worrisome as EB is a mutagen and probable carcinogen. Five alternative stains were evaluated for DNA detection, safety, cost, and ease of use: BlueView, methylene blue, Carolina Blu, DAPI (4',6-diamidino-2-phenylindole dihydrochloride:hydrate), and SYBR Green I. BlueView, Carolina Blu, and methylene blue are not sensitive enough to detect the microgram amounts of DNA used in many procedures. However, DAPI and SYBR Green I are good staining alternatives to ethidium bromide in that they have similar sensitivity and are both easy to use. SYBR Green I is more expensive than EB or DAPI; however, the limited safety data suggest that SYBR Green I is the safest stain.
ISSN:0021-9584
1938-1328
DOI:10.1021/ed080p1292