Formulation of Liposome using Sappan wood (Caesalpinia Sappan L.) Ethyl acetate Fraction as an activate compound

The purpose of this study is to determine the characteristics of liposome containing Sappan wood ethyl acetate fraction (SWEAF) as an active compound. Liposome was prepared by using two methods: Thin Layer Hydration (TLH) and Reversed Phase Evaporation (REV) methods. Liposome composition was 1 part of the SWEAF and 6 parts of the liposome component (phosphatidyl choline and cholesterol with an equally large molar ratio). Both methods produce red-colored liposome dispersion. When examined with transmission electrone microscope, both methods yielded a spherical and unilamellar liposome. The TLH method produces liposome with a particle size of 2,304.5 ± 528.9 nm. The REV method yields a liposome with a particle size of 2,643.5 ± 350.8 nm. The polydispersity index shows the distribution of liposome size. The TLH method produces liposome with a polydispersity index of 1,490 ± 0.284. The REV method produces liposome with a polydispersity index of 1.987 ± 0.901.