Facile synthesis of polylactide coarse microspheres as artificial antigen-presenting cells

Magnetic poly( l -lactide) coarse microspheres were synthesized via an emulsion method followed by chemical etching. Subsequently, anti-CD3 and anti-CD28 antibodies were attached to the microspheres via an avidin-biotin amplification system as artificial antigen-presenting cells. The activation of T cells was enhanced significantly due to the surface topographic structure and increased signal molecule amount. Magnetic poly( l -lactide) coarse microspheres as artificial antigen-presenting cells were synthesized via simple chemical etching and antibody immobilization.