Determination of Hyaluronic Acid in Aqueous Solutions Using Air as an Oxidant

The development of new methods for the control of preparations widely used in cosmetics and medicine is a task of high current importance. Hyaluronic acid is one of the most often used components of anti-age cosmetics; its action and impact on the human skin depends on the concentration in the solution. The known methods of the determination of the concentration of hyaluronic acid are expensive, require complex sample preparation, and cannot be used in routine analyses. In this work, for the rapid determination of the concentration of hyaluronic acid in solutions used in the production of skin cosmetics, we propose the method of oxythermography, which does not require chemical reagents. The oxidant is air oxygen. The absolute limit of detection for hyaluronic acid is about 4 µg for a sample volume of 5 µL.