Effect of Embryo Holding on Bovine Oocyte Maturation outside the Incubator

Maintenance of immature oocytes before the onset of puberty help to do next manipulation. The aim of study was to use Embryo Holding with and without the use of the incubator for transfering oocytes to the laboratory and examining effects on oocytes maturation.Cow ovaries were collected from slaughterhouse animaland 6 groups (EHT) of them were replaced on the Embryo Holding (Syngro@) and kept in darkness in a styrofoam container with thermo packs at 4°C, 22°C, 38.5°C for 12, 24 hours ( EHT-12h, EHT-24h ).One group (C1) was replaced on the standard medium IVM.After staining , the highest percentage of oocytes maturation was 92/2 % in the C1 while it had significantly different from all groups EHT (P≤0/05). In the EHT-12h numerically had the highest percentage of maturation in 22°C (80%) and they didn’t have significant difference with groups EHT-12h (P>0/05). In EHT-24h, the highest percentage of oocytes maturation were in 38.5°C (54.3%) and they had statistically significant difference with 4°C (P≤0/05). Embryo Holding media in our study showed that can be helpful for maintenance of immature oocytes until 12 hours at room temperature and without incubation.