Pseudomonas fluorescens AK Lipase Immobilization on MCM-48-Type Mesoporous Support in the Presence of Ionic Liquid

Pseudomonas fluorescens AK lipase was in situ immobilized on MCM-48-type mesoporous material employing the ionic liquid 1-hexadecyl-3-methylimidazolium chloride as a template for the inorganic material synthesis and also as an additive in the enzyme immobilization for lipase as a catalyst in chemical reactions--making it possible to reuse it. Storage stability, operational, and the potential for application in ester synthesis studies were performed. The support synthesis and AK lipase immobilization processes were efficient, presenting a 664% esterification yield and a 578 U/g enzymatic activity with 4 continuous reuse cycles, in addition to a conversion to the ester synthesis of ethyl and geranyl oleates of 14 and 18%, respectively. Thus, the results presented demonstrate the potential of using this mesoporous material in the enzymatic immobilization process.