Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics

Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics

We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ). FISSEQ-BOINC exhibits different properties from BOINC, which relies on bulk nuc...

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Veröffentlicht in:arXiv.org 2014-04
Hauptverfasser: Marblestone, Adam H, Daugharthy, Evan R, Kalhor, Reza, Peikon, Ian D, Kebschull, Justus M, Shipman, Seth L, Mishchenko, Yuriy, Lee, Je Hyuk, Kording, Konrad P, Boyden, Edward S, Zador, Anthony M, Church, George M
Format: Artikel
Sprache:eng
Schlagworte:
Annotations
Brain
Fluorescence
Mapping
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Zusammenfassung:We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ). FISSEQ-BOINC exhibits different properties from BOINC, which relies on bulk nucleic acid sequencing. FISSEQ-BOINC could become a scalable approach for mapping whole-mammalian-brain connectomes with rich molecular annotations.
ISSN:2331-8422