Isolation, identification and molecular characterization of virulent avian infectious bronchitis Virus in Khyber Pakhtunkhwa, Pakistan

Infectious bronchitis virus (IBV) of the Corona viridae family is a single stranded RNA virus; a causative agent of respiratory and urogenital tract diseases in chickens. This pathogen causes huge economic losses throughout the world where chickens are produced for commercial purposes. Embryonated e...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Pure and applied biology 2018-06, Vol.7 (2), p.435-442
Hauptverfasser: Shabina, Sarzameen, ul Hayat, Shams, Gul, Saira, Gul, Saleha, Haseena, Attaullah
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Infectious bronchitis virus (IBV) of the Corona viridae family is a single stranded RNA virus; a causative agent of respiratory and urogenital tract diseases in chickens. This pathogen causes huge economic losses throughout the world where chickens are produced for commercial purposes. Embryonated egg inoculation was used for detection and amplification of IBV RNA, (specific pathogen free) SPF eggs (n=50, 9-11 days old) were inoculated with IBV suspected samples. After incubating the eggs for 72-144hours, the infectious allantoic fluids were harvested when signs of curling, stunting, dwarfing and death of the embryos appeared after inoculation. Viral RNA used in the RT-PCR was extracted from virus containing allantoic fluid and reverse transcribed to cDNA for detection of IBV and their serotypes. PCR products were electrophoresed to visualize the results. A high percentage (36/50eggs) of the eggs (P
ISSN:2304-2478
2304-2478
DOI:10.19045/bspab.2018.70054