Isolation, identification and molecular characterization of virulent avian infectious bronchitis Virus in Khyber Pakhtunkhwa, Pakistan
Infectious bronchitis virus (IBV) of the Corona viridae family is a single stranded RNA virus; a causative agent of respiratory and urogenital tract diseases in chickens. This pathogen causes huge economic losses throughout the world where chickens are produced for commercial purposes. Embryonated e...
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Veröffentlicht in: | Pure and applied biology 2018-06, Vol.7 (2), p.435-442 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Infectious bronchitis virus (IBV) of the Corona viridae family is a single stranded RNA virus; a causative agent of respiratory and urogenital tract diseases in chickens. This pathogen causes huge economic losses throughout the world where chickens are produced for commercial purposes. Embryonated egg inoculation was used for detection and amplification of IBV RNA, (specific pathogen free) SPF eggs (n=50, 9-11 days old) were inoculated with IBV suspected samples. After incubating the eggs for 72-144hours, the infectious allantoic fluids were harvested when signs of curling, stunting, dwarfing and death of the embryos appeared after inoculation. Viral RNA used in the RT-PCR was extracted from virus containing allantoic fluid and reverse transcribed to cDNA for detection of IBV and their serotypes. PCR products were electrophoresed to visualize the results. A high percentage (36/50eggs) of the eggs (P |
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ISSN: | 2304-2478 2304-2478 |
DOI: | 10.19045/bspab.2018.70054 |