A lateral flow channel immunoassay combining a particle binding zone geometry with nanoparticle labelling amplification

A lateral flow channel immunoassay combining a particle binding zone geometry with nanoparticle labelling amplification

•Channel flow design with reaction area: sample volume >20 times the open channel immunoassay format.•Simple channel flow design for samples of ≥10 μL.•Reaction zone of silica particles with covalently linked capture antibodies, combined with non-direct fluorescent nanoparticles.•Strong fluoresce...

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Veröffentlicht in:Sensors and actuators. B, Chemical Chemical, 2018-06, Vol.262, p.1-8
Hauptverfasser: Engels, Jan F., Henderson, Cassi J., Daly, Ronan, Renneberg, Reinhard, Hall, Elizabeth A.H.
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Analytical chemistry
Antibodies
Binding
Chemical compounds
Fluorescein
Fluorescence
Format
Geometry
Immunoassay
Labeling
Lateral flow microfluidics
Molecular chains
Nanoparticles
Non-direct fluorescence
Optimization
Polyclonal antibodies
Signal generation
Silicon dioxide
Sodium hydroxide
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Beschreibung
Zusammenfassung:•Channel flow design with reaction area: sample volume >20 times the open channel immunoassay format.•Simple channel flow design for samples of ≥10 μL.•Reaction zone of silica particles with covalently linked capture antibodies, combined with non-direct fluorescent nanoparticles.•Strong fluorescent signal generated from fluorescent pre-cursor particles.•Model system of RbIgG/Gt-α RbIgG showed good linearity for detection of
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2018.01.213