The use of PCR applications for assessment of Clostridium botulinum presence in the culture environment and fish samples in the conditions of open system production from Romanian fisheries

A rapid expansion in world aquaculture has taken place in recent years due to its highest potential to answer to the consumers demands on healthy food products. The application of biosecurity in aquaculture production systems and the safety of fish and fish products, in the context of climate changes, requires investigations of the production systems and solutions for processing and preservation technologies of fish products. PCR methods will be developed using the procedure of genes fragments amplification for Clostridium botulinum van Ermengem, 1896 species producing toxins A, B, E and F from isolates of C. botulinum using extract broth TGPY (culture medium used for proteolytic strains) and TPGYT (culture medium used for non-proteolytic strains of C. botulinum). The results will contribute to the integral development of food safety management and the quality of fish on the fish chain focused on the Cyprinidae for humans consumption.