SYNERGISTIC EFFECT OF WALNUT AND BLACK CHOKEBERRY MIXTURE ON ANTI-LIPID PEROXIDATION IN D-GALACTOSE INDUCED AGING MODEL

Background and objectives: Many studies mentioned the cellular damage caused by reactive oxygen species(ROS) could be aging. D-galactose causes aging-related changes and oxidative stress in the organism. Lipids are the first target of free radicals, and lipid peroxidation related the aging process. Walnut kernels (Juglans regia L.) contains antioxidant phenolic compounds. Many studies showed that walnut phenolics belong to ellagitannin, or hydrolyzable tannins related antioxidant activities. Chokeberry(Aronia melanocarpa) is one of the richest sources of polyphenols, especially anthocyanin among other fruits. Previous studies showed that polyphenols of chokeberry have antioxidant and anti-inflammatory activities. In this study, we mainly investigated that synergistic effect of walnut and chokeberry mixture on anti-lipid peroxidation of the serum and various tissues in D-galactose induced aging model. Methods: Thirty-five Balb/c mice(8 weeks of age) were divided into five groups(n=7 for each group): the normal control group(NC), D-galactose control group(DC), D-galactose with chokeberry diet group(CHD), D-galactose with walnut diet(WD), and D-galactose with walnut and chokeberry mixture diet(WCH). All groups were intraperitoneally infected with D-galactose(120mg/kg) daily for eight weeks, except for NC group which was treated with saline. Levels of malondialdehyde(MDA) in serum, liver, brain, and kidney were determined by according assay kits in D-galactose treated mice. Results: It was found that the serum, hepatic, and brain MDA level in DC groups were higher than the NC group(p