EFFECTS OF BIOTIN STATUS ON GLYCEMIC CONTROL IN RATS

Background and objectives: Biotin, as a coenzyme of carboxylases, plays an important role in the process of neoglycogenesis, amino acid decomposition, fatty acid synthesis and energy metabolism in humans and mammals. In recent studies, it has showed that large dose of biotin supplement can be taken as drug to treat hyperglycemia, through potential mechanism of mediating gene regulation of histone biotinylation. The main source of body biotin are main from dietary intake and intestinal bacterial synthesizing, so biotin deficiency is rare, the relationship between biotin status and glycemic control should be studied. Methods: 60 healthy SD rats (F/M half to half) weighting (100±10) grams were fed with non-biotin diet with raw egg white replacing casein to induce biotin deficiency model (NB), then divided into 6 test groups, daily oral given 0, 2, 4, 10 µg biotin, 1g resistance dextrin (RD, as a stimulant of intestinal source) or mixture of 4 µg biotin and 1g RD respectively. Additional 20 rats fed with diet based on AIN-93 as blank control. After 8 weeks intervention term, blood biotin and 3HIA-carnitine, glucose and lipid level, organ pathology, intestinal bacterial were evaluated. Results: From the 2nd day of NB diet feeding, serum biotin immediately appeared lower than the control group, while 3HIA-carnitine increased (P