An Enhanced Direct Competitive Immunoassay for the Detection of Kanamycin and Tobramycin in Milk Using Multienzyme-Particle Amplification

An Enhanced Direct Competitive Immunoassay for the Detection of Kanamycin and Tobramycin in Milk Using Multienzyme-Particle Amplification

Kanamycin (Kan) and tobramycin (Tob) are widely found in many foods of animal origin, including milk. More rapid, simple, and sensitive methods are urgently needed to monitor antibiotic residues in milk. An enhanced direct competitive enzyme-linked immunosorbent assay (dcELISA) based on gold nanopar...

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Veröffentlicht in:Food analytical methods 2018-08, Vol.11 (8), p.2066-2075
Hauptverfasser: Jiang, Ling, Wei, Dali, Zeng, Kun, Shao, Jie, Zhu, Fang, Du, Daolin
Format: Artikel
Sprache:eng
Schlagworte:
Analytical Chemistry
Antibiotics
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Contaminants
Cross-reactivity
Economic conditions
Enzyme-linked immunosorbent assay
Food Science
Gold
Immunoassay
Kanamycin
Microbiology
Milk
Monoclonal antibodies
Nanoparticles
Optimization
Peroxidase
Reproducibility
Tobramycin
Trace contaminants
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Zusammenfassung:Kanamycin (Kan) and tobramycin (Tob) are widely found in many foods of animal origin, including milk. More rapid, simple, and sensitive methods are urgently needed to monitor antibiotic residues in milk. An enhanced direct competitive enzyme-linked immunosorbent assay (dcELISA) based on gold nanoparticles (AuNPs)/horse radish peroxidase-Kan (HRP-Kan) was developed. A monoclonal antibody (Mab) against Kan was developed by classic hybridoma technology. The Mab had higher cross-reactivity with Tob (99.07%) and no cross-reactivity with other related antibiotics (
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-018-1185-2