Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts
Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2007-09, Vol.73 (18), p.5949-5950 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5950 |
|---|---|
| container_issue | 18 |
| container_start_page | 5949 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 73 |
| creator | Miller, Kathryn M Sterling, Charles R |
| description | Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection. |
| doi_str_mv | 10.1128/AEM.00668-07 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_highw</sourceid><recordid>TN_cdi_proquest_journals_205977500</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20617048</sourcerecordid><originalsourceid>FETCH-LOGICAL-c521t-d0da7a79949ec9b85e64d7a0b7fc1af2e38ae30090c1af6a9d930b517dccbb1c3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhiMEokvhxhkiJDiRMk78eUGqllKQloJaerYmjrPrKolbO2m1_x6HXdHChdNoNI9ezcyTZS8JHBFSyg_HJ9-OADiXBYhH2YKAkgWrKv44WwAoVZQlhYPsWYxXAECBy6fZARGcUk75Iju_sEN0o7t14zb3bX5m42ib_MfyPHdDPm5s_smO1ozOD_N45e_ys6mvbYhze-owNA7zDvu6S3W5jWN8nj1psYv2xb4eZpefT34uvxSr76dfl8erwrCSjEUDDQoUSlFljaols5w2AqEWrSHYlraSaKt0AswtR9WoCmpGRGNMXRNTHWYfd7nXU93bxthhDNjp6-B6DFvt0em_J4Pb6LW_1SUIqoClgHf7gOBvpnS47l00tutwsH6KmsuSCSXgv2AJnAigMoFv_gGv_BSG9IXEMCUEgznt_Q4ywccYbPtnZQJ6VqqTUv1bqQaR8FcPz7yH9w4T8HYPYDTYtQEH4-I9p0BKyh4st3HrzZ0LVmPsNdpei0oTqVkykaDXO6hFr3EdUtDlRQkkiZDAqWTVL9JzvVs</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205977500</pqid></control><display><type>article</type><title>Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts</title><source>MEDLINE</source><source>American Society for Microbiology Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Miller, Kathryn M ; Sterling, Charles R</creator><creatorcontrib>Miller, Kathryn M ; Sterling, Charles R</creatorcontrib><description>Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.00668-07</identifier><identifier>PMID: 17644646</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Biological and medical sciences ; Cysts ; DNA Primers - chemistry ; DNA Primers - genetics ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Fundamental and applied biological sciences. Psychology ; Giardia lamblia ; Giardia lamblia - genetics ; Giardia lamblia - isolation & purification ; Giardiasis - diagnosis ; Methods ; Microbiology ; Parasitic protozoa ; Pathogens ; Polymerase Chain Reaction - methods ; Sensitivity and Specificity</subject><ispartof>Applied and Environmental Microbiology, 2007-09, Vol.73 (18), p.5949-5950</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Sep 2007</rights><rights>Copyright © 2007, American Society for Microbiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-d0da7a79949ec9b85e64d7a0b7fc1af2e38ae30090c1af6a9d930b517dccbb1c3</citedby><cites>FETCH-LOGICAL-c521t-d0da7a79949ec9b85e64d7a0b7fc1af2e38ae30090c1af6a9d930b517dccbb1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074905/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074905/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19088458$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17644646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, Kathryn M</creatorcontrib><creatorcontrib>Sterling, Charles R</creatorcontrib><title>Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cysts</subject><subject>DNA Primers - chemistry</subject><subject>DNA Primers - genetics</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Giardia lamblia</subject><subject>Giardia lamblia - genetics</subject><subject>Giardia lamblia - isolation & purification</subject><subject>Giardiasis - diagnosis</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Parasitic protozoa</subject><subject>Pathogens</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sensitivity and Specificity</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhxhkiJDiRMk78eUGqllKQloJaerYmjrPrKolbO2m1_x6HXdHChdNoNI9ezcyTZS8JHBFSyg_HJ9-OADiXBYhH2YKAkgWrKv44WwAoVZQlhYPsWYxXAECBy6fZARGcUk75Iju_sEN0o7t14zb3bX5m42ib_MfyPHdDPm5s_smO1ozOD_N45e_ys6mvbYhze-owNA7zDvu6S3W5jWN8nj1psYv2xb4eZpefT34uvxSr76dfl8erwrCSjEUDDQoUSlFljaols5w2AqEWrSHYlraSaKt0AswtR9WoCmpGRGNMXRNTHWYfd7nXU93bxthhDNjp6-B6DFvt0em_J4Pb6LW_1SUIqoClgHf7gOBvpnS47l00tutwsH6KmsuSCSXgv2AJnAigMoFv_gGv_BSG9IXEMCUEgznt_Q4ywccYbPtnZQJ6VqqTUv1bqQaR8FcPz7yH9w4T8HYPYDTYtQEH4-I9p0BKyh4st3HrzZ0LVmPsNdpei0oTqVkykaDXO6hFr3EdUtDlRQkkiZDAqWTVL9JzvVs</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Miller, Kathryn M</creator><creator>Sterling, Charles R</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070901</creationdate><title>Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts</title><author>Miller, Kathryn M ; Sterling, Charles R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-d0da7a79949ec9b85e64d7a0b7fc1af2e38ae30090c1af6a9d930b517dccbb1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cysts</topic><topic>DNA Primers - chemistry</topic><topic>DNA Primers - genetics</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Giardia lamblia</topic><topic>Giardia lamblia - genetics</topic><topic>Giardia lamblia - isolation & purification</topic><topic>Giardiasis - diagnosis</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Parasitic protozoa</topic><topic>Pathogens</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, Kathryn M</creatorcontrib><creatorcontrib>Sterling, Charles R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, Kathryn M</au><au>Sterling, Charles R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>73</volume><issue>18</issue><spage>5949</spage><epage>5950</epage><pages>5949-5950</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>17644646</pmid><doi>10.1128/AEM.00668-07</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2007-09, Vol.73 (18), p.5949-5950 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_proquest_journals_205977500 |
| source | MEDLINE; American Society for Microbiology Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Cysts DNA Primers - chemistry DNA Primers - genetics DNA, Protozoan - chemistry DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology Giardia lamblia Giardia lamblia - genetics Giardia lamblia - isolation & purification Giardiasis - diagnosis Methods Microbiology Parasitic protozoa Pathogens Polymerase Chain Reaction - methods Sensitivity and Specificity |
| title | Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T13%3A25%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_highw&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitivity%20of%20Nested%20PCR%20in%20the%20Detection%20of%20Low%20Numbers%20of%20Giardia%20lamblia%20Cysts&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Miller,%20Kathryn%20M&rft.date=2007-09-01&rft.volume=73&rft.issue=18&rft.spage=5949&rft.epage=5950&rft.pages=5949-5950&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.00668-07&rft_dat=%3Cproquest_highw%3E20617048%3C/proquest_highw%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205977500&rft_id=info:pmid/17644646&rfr_iscdi=true |