Sensitivity of Nested PCR in the Detection of Low Numbers of Giardia lamblia Cysts

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

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Veröffentlicht in:	Applied and Environmental Microbiology 2007-09, Vol.73 (18), p.5949-5950
Hauptverfasser:	Miller, Kathryn M, Sterling, Charles R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cysts DNA Primers - chemistry DNA Primers - genetics DNA, Protozoan - chemistry DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology Giardia lamblia Giardia lamblia - genetics Giardia lamblia - isolation & purification Giardiasis - diagnosis Methods Microbiology Parasitic protozoa Pathogens Polymerase Chain Reaction - methods Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.00668-07