Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate

Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant througho...

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Veröffentlicht in:	Applied and Environmental Microbiology 2007-02, Vol.73 (4), p.1349-1354
Hauptverfasser:	Du, Meng, Chen, Jixiang, Zhang, Xiaohua, Li, Aijuan, Li, Yun, Wang, Yingeng
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriology Biological and medical sciences Cell culture Cellular biology Culture Media Edwardsiella tarda Edwardsiella tarda - isolation & purification Edwardsiella tarda - pathogenicity Fish Diseases - microbiology Fundamental and applied biological sciences. Psychology Marine Microbial Ecology Microbial Viability Microbiology Pathology Scanning electron microscopy Scophthalmus maximus Virulence Water Microbiology
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Beschreibung
Zusammenfassung:	Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.02243-06