Genetic identification of a putative vinyl choloride reductase in Dehalococcoides sp. strain BAV1

Genetic identification of a putative vinyl choloride reductase in Dehalococcoides sp. strain BAV1

Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase ge...

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Veröffentlicht in:Applied and environmental microbiology 2004-10, Vol.70 (10), p.6347
Hauptverfasser: Krajmalnik-Brown, Rosa, Holscher, Tina, Thomson, Ivy N, Saunders, F Michael
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria
Enzymes
Genetics
Polyvinyl chloride
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Zusammenfassung:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures. [PUBLICATION ABSTRACT]
ISSN:0099-2240
1098-5336