Detection of an arbuscular mycorrhizal fungus in roots of different plant species with the PCR

PCR was used with the primer pair VANS1-NS21 to detect the arbuscular mycorrhizal fungus Glomus intraradices (commercial inoculum source) on roots of lettuce, zinnia, leek, pepper, and endive plants. The appropriate amplification product was obtained directly from roots without DNA extraction and pu...

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Veröffentlicht in:	Applied and Environmental Microbiology 1995-07, Vol.61 (7), p.2809-2810
Hauptverfasser:	Di Bonito, R. (INN-BIOAG, Rome, Italy.), Elliott, M.L, Jardin, E.A. des
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions ALLIUM AMPELOPRASUM AMPLIFICATION CHAINE POLYMERASE Biological and medical sciences CAPSICUM ANNUUM CICHORIUM ENDIVIA DNA, Fungal - analysis Economic plant physiology Fundamental and applied biological sciences. Psychology Fungi Fungi - genetics Fungi - isolation & purification GLOMUS Glomus intraradices LACTUCA SATIVA MICORRIZAS ARBUSCULARES VESICULARES MYCORHIZE A VESICULE ET ARBUSCULE Plants - microbiology Polymerase Chain Reaction RACINE RAICES REACCION DE CADENAS DE POLIMERASA Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Vegetables ZINNIA
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Beschreibung
Zusammenfassung:	PCR was used with the primer pair VANS1-NS21 to detect the arbuscular mycorrhizal fungus Glomus intraradices (commercial inoculum source) on roots of lettuce, zinnia, leek, pepper, and endive plants. The appropriate amplification product was obtained directly from roots without DNA extraction and purification
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.61.7.2809-2810.1995