The role of Tom70 in cardiac microvascular endothelial cell damage induced by high fat and high glucose

Objective To investigate the effect of high glycolipid on mouse cardiac microvascular endothelial cells (MCMECs), clarify the role of Tom70 (translocase of the outer mitochondrial membrane 70, Tom70) in it, and explore the related mechanism. Methods MCMECs cultured with normal glucose medium were divided into normal glucose group (NG, 5.5mmol/L), high glucose group (HG, 25mmo1/L) and HG combined with high fat group (HG+HF, glucose concentration 25mmo1/L, 500μmol/L, 16h). Then, the expression of Tom70 in MCMECs was knocked down by siRNA, and the HG+HF group was further divided into vehicle group, Scramble siRNA group and Tom70-siRNA group. To further confirm the specific mechanism of Tom70 in MCMEC injury induced by high glycolipid, Tom70-siRNA group was subgrouped into N-acetylcysteine (NAC)-free group and NAC-containing group. Accordingly, the apoptosis levels were measured by flow cytometer, the generation of nitric oxide (NO) was detected by ELISA kit, the expressions of Tom70 were determined by immunofluo