Bisfenol A'nın Tuba Uterina'da Neonatal Dönemde Oluşturduğu Değişikliklere Karşı Melatonin'in Olası Etkisi

Objective: The purpose is to investigate the possible protective effect of melatonin against the alterations of Bisphenol A (BPA) which has caused an injury in humans and animals through food and beverage packaging materials, on tuba uterina in neonatal period by histopathological examinations. Material and Methods: 24 Wistar albino neonatal female rats were divided into 4 groups, each including 6 rats: 1. Group: Control, 2. Group: BPA (100 mg/kg), 3. Group: Melatonin (10mg/kg), 4. Group: BPA+Melatonin (100mg/kg+10mg/kg). The vaginal openings of subjects were measured and smear samples were examined. At the end of the 70 days experiment, tuba uterina tissues were taken from subjects which were sacrificed under high-dose anesthesia. Sections were stained by Hematoxylin-Eosine and Masson’s trichrome. The data obtained by measuring subjects’ body weight, epithelial height and wall thickness were also supported statistically. Results: As a result, normal tuba uterina histological findings were seen in control and melatonin groups. Also it was found that BPA administration has moved puberty to earlier time whereas BPA and Melatonin administration has delayed the puberty by assessing vaginal opening and smear determination. Following the histological examinations on tuba uterina tissues, it was determined that mucosal residues formed cystic structures by reaching into lamina propria, and epithelium sporadically became stratified as a result of BPA administration. These findings were descended to the lowest levels by melatonin administrations in BPA+Melatonin group. It was also observed that the cilia were merged in some areas while to- tally disappeared in others. Thickening of the serosal layer was described as the most remarkable finding. This finding is also statistically supported by measuring wall thickness. Conclusion: It was concluded that melatonin administration provides a significant protection against BPA-related changes.