Role of the PufB and PufA C-Terminal Extensions in the Assembly of Rhodospirillum rubrum Light-Harvesting Antenna

The light-harvesting antenna (LH) proteins of Rhodospirillum rubrum are encoded by pufA and pufB with C-terminal extensions not present in the mature proteins. Point mutations were introduced into pufB, pufA, and both pufB and pufA so that proteins equivalent to the mature proteins were encoded. The...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Current microbiology 2010-04, Vol.60 (4), p.301-306
Hauptverfasser:	Butzin, Nicholas C, Benson, Jodi A, Merchant, Daniel J, Collins, Mary Lynne Perille
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Biotechnology Life Sciences Light-Harvesting Protein Complexes - genetics Light-Harvesting Protein Complexes - metabolism Microbiology Mutagenesis, Site-Directed Mutation Point Mutation Protein Binding Protein Interaction Mapping Proteins Rhodospirillum rubrum - physiology Temperature effects
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The light-harvesting antenna (LH) proteins of Rhodospirillum rubrum are encoded by pufA and pufB with C-terminal extensions not present in the mature proteins. Point mutations were introduced into pufB, pufA, and both pufB and pufA so that proteins equivalent to the mature proteins were encoded. The mutants with these truncated proteins produced 3-30% of the wild-type level of LH. These findings suggest that the C-terminal extensions are required for wild-type levels of LH.
ISSN:	0343-8651 1432-0991
DOI:	10.1007/s00284-009-9541-5