Antioxidant, Antigenotoxic, Antimicrobial Activities and Phytochemical Analysis of Dianthus carmelitaru Rezzan Aliyazicioglu, Selim Demir, Merve Badem, Sila Ozlem Sener, Nuriye Korkmaz, Elif Ayazoglu Demir, Ufuk Ozgen, Sengul Alpay Karaoglu and Yuksel Aliyazicioglu

In this study, we investigated the phytochemical composition, antioxidant, antimicrobial, and antigenotoxic properties of the aqueous extract of Dianthus carmelitarum for the first time. The phenolic and volatile compounds, antioxidant, antimicrobial and antigenotoxic activities of the extract were determined by HPLC and SPME-GC-FID/MS, spectrophotometric, agar well diffusion methods and comet assay, respectively. The polyphenolic content and ferric reducing power values of the extract were found 12.6 ± 0.27 mg gallic acid and 238 ± 2.89 μM trolox equivalents per g sample, respectively. Syringaldehyde and chlorogenic acid were detected as major phenolic compounds, while terpenes were determined as major volatile compound. Dianthus carmelitarum extract especially exhibited moderate antimicrobial activity against Mycobacterium smegmatis. Extract reduced H 2O 2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to positive control (only 20 μM H 2O 2 treatment). Dianthus carmelitarum can be considered in the food, cosmetic, and drug industries due to its antioxidant, antimicrobial, and antigenotoxic activities. Key words: Antimicrobial activity; antioxidant effect; chromatography; Dianthus carmelitarum; genotoxic stress. © 2017 ACG Publications. All rights reserved.