Effects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos)
The present study investigated possible benefits of magnesium ion (as MgCl 2 ) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing EDTA we...
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| Veröffentlicht in: | Pharmacology research & perspectives 2018-06, Vol.6 (3) |
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| creator | Ajilore, Bamidele S. Alli, Adetayo A. Oluwadairo, Tolulope O. |
| description | The present study investigated possible benefits of magnesium ion (as MgCl
2
) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing
EDTA
were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl
2
. Acetylcholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase activities were evaluated. Results showed that Chlorpyrifos significantly (
P
|
| doi_str_mv | 10.1002/prp2.401 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2049614467</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2049614467</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1007-fa9660360725a63ba83d6665bd5bef392652901f624083e6cccaeef214c1ec173</originalsourceid><addsrcrecordid>eNpNkMFKAzEURYMoWGrBTwi4qYupL8lMprMspVahoIu6DplM0qa0SZpMhf6N3-KXOWNduHqXx-G-dy9C9wQmBIA-hRjoJAdyhQYUCpqREsrrf_oWjVLaAQAhHcXoAB0XxmjVJuwNPsiN08meDlht9z7aRmPvsHXfX5-2jb7b-r3tiFZHmTSWrsGz9TvudfA2eWfdBtdn7ONGOh-2PoWtbDUe_9qFc7TGp8c7dGPkPunR3xyij-fFev6Srd6Wr_PZKlNdlDIzsuIcGIeSFpKzWk5Zwzkv6qaotWEV5QWtgBhOc5gyzZVSUmtDSa6IVqRkQ_Rw8Q3RH0_d12LnT9F1JwWFvOIkz3lPjS-Uij6lqI0I0R5kPAsCou9U9J2Kvq0fWAtq3A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2049614467</pqid></control><display><type>article</type><title>Effects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos)</title><source>Wiley Online Library - AutoHoldings Journals</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>Ajilore, Bamidele S. ; Alli, Adetayo A. ; Oluwadairo, Tolulope O.</creator><creatorcontrib>Ajilore, Bamidele S. ; Alli, Adetayo A. ; Oluwadairo, Tolulope O.</creatorcontrib><description>The present study investigated possible benefits of magnesium ion (as MgCl
2
) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing
EDTA
were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl
2
. Acetylcholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase activities were evaluated. Results showed that Chlorpyrifos significantly (
P
< .05) reduced the levels of cholinesterase both in plasma and on red blood cells. Red blood cells Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase were also significantly (
P
< .05) reduced by chlorpyrifos while MgCl
2
counteracted effects of chlorpyrifos with significant (
P
< .05) increase in the levels of cholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase. We concluded that MgCl
2
neutralized effects of chlorpyrifos by promoting normal
ATP
ase activities and inhibiting release of acetylcholine from cell.</description><identifier>ISSN: 2052-1707</identifier><identifier>EISSN: 2052-1707</identifier><identifier>DOI: 10.1002/prp2.401</identifier><language>eng</language><publisher>Bognor Regis: John Wiley & Sons, Inc</publisher><subject>Adenosine triphosphate ; Blood ; Chloride ; Enzymes ; Farms ; Insecticides ; Pesticides ; Pharmacology ; Phosphate esters ; Phosphorylation ; Poisoning ; Studies</subject><ispartof>Pharmacology research & perspectives, 2018-06, Vol.6 (3)</ispartof><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1007-fa9660360725a63ba83d6665bd5bef392652901f624083e6cccaeef214c1ec173</citedby><cites>FETCH-LOGICAL-c1007-fa9660360725a63ba83d6665bd5bef392652901f624083e6cccaeef214c1ec173</cites><orcidid>0000-0003-1681-5880</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Ajilore, Bamidele S.</creatorcontrib><creatorcontrib>Alli, Adetayo A.</creatorcontrib><creatorcontrib>Oluwadairo, Tolulope O.</creatorcontrib><title>Effects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos)</title><title>Pharmacology research & perspectives</title><description>The present study investigated possible benefits of magnesium ion (as MgCl
2
) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing
EDTA
were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl
2
. Acetylcholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase activities were evaluated. Results showed that Chlorpyrifos significantly (
P
< .05) reduced the levels of cholinesterase both in plasma and on red blood cells. Red blood cells Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase were also significantly (
P
< .05) reduced by chlorpyrifos while MgCl
2
counteracted effects of chlorpyrifos with significant (
P
< .05) increase in the levels of cholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase. We concluded that MgCl
2
neutralized effects of chlorpyrifos by promoting normal
ATP
ase activities and inhibiting release of acetylcholine from cell.</description><subject>Adenosine triphosphate</subject><subject>Blood</subject><subject>Chloride</subject><subject>Enzymes</subject><subject>Farms</subject><subject>Insecticides</subject><subject>Pesticides</subject><subject>Pharmacology</subject><subject>Phosphate esters</subject><subject>Phosphorylation</subject><subject>Poisoning</subject><subject>Studies</subject><issn>2052-1707</issn><issn>2052-1707</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpNkMFKAzEURYMoWGrBTwi4qYupL8lMprMspVahoIu6DplM0qa0SZpMhf6N3-KXOWNduHqXx-G-dy9C9wQmBIA-hRjoJAdyhQYUCpqREsrrf_oWjVLaAQAhHcXoAB0XxmjVJuwNPsiN08meDlht9z7aRmPvsHXfX5-2jb7b-r3tiFZHmTSWrsGz9TvudfA2eWfdBtdn7ONGOh-2PoWtbDUe_9qFc7TGp8c7dGPkPunR3xyij-fFev6Srd6Wr_PZKlNdlDIzsuIcGIeSFpKzWk5Zwzkv6qaotWEV5QWtgBhOc5gyzZVSUmtDSa6IVqRkQ_Rw8Q3RH0_d12LnT9F1JwWFvOIkz3lPjS-Uij6lqI0I0R5kPAsCou9U9J2Kvq0fWAtq3A</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Ajilore, Bamidele S.</creator><creator>Alli, Adetayo A.</creator><creator>Oluwadairo, Tolulope O.</creator><general>John Wiley & Sons, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0003-1681-5880</orcidid></search><sort><creationdate>201806</creationdate><title>Effects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos)</title><author>Ajilore, Bamidele S. ; Alli, Adetayo A. ; Oluwadairo, Tolulope O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1007-fa9660360725a63ba83d6665bd5bef392652901f624083e6cccaeef214c1ec173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenosine triphosphate</topic><topic>Blood</topic><topic>Chloride</topic><topic>Enzymes</topic><topic>Farms</topic><topic>Insecticides</topic><topic>Pesticides</topic><topic>Pharmacology</topic><topic>Phosphate esters</topic><topic>Phosphorylation</topic><topic>Poisoning</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ajilore, Bamidele S.</creatorcontrib><creatorcontrib>Alli, Adetayo A.</creatorcontrib><creatorcontrib>Oluwadairo, Tolulope O.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Pharmacology research & perspectives</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ajilore, Bamidele S.</au><au>Alli, Adetayo A.</au><au>Oluwadairo, Tolulope O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos)</atitle><jtitle>Pharmacology research & perspectives</jtitle><date>2018-06</date><risdate>2018</risdate><volume>6</volume><issue>3</issue><issn>2052-1707</issn><eissn>2052-1707</eissn><abstract>The present study investigated possible benefits of magnesium ion (as MgCl
2
) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing
EDTA
were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl
2
. Acetylcholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase activities were evaluated. Results showed that Chlorpyrifos significantly (
P
< .05) reduced the levels of cholinesterase both in plasma and on red blood cells. Red blood cells Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase were also significantly (
P
< .05) reduced by chlorpyrifos while MgCl
2
counteracted effects of chlorpyrifos with significant (
P
< .05) increase in the levels of cholinesterase, Na
+
/K
+
ATP
ase and Ca
2+
ATP
ase. We concluded that MgCl
2
neutralized effects of chlorpyrifos by promoting normal
ATP
ase activities and inhibiting release of acetylcholine from cell.</abstract><cop>Bognor Regis</cop><pub>John Wiley & Sons, Inc</pub><doi>10.1002/prp2.401</doi><orcidid>https://orcid.org/0000-0003-1681-5880</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Adenosine triphosphate Blood Chloride Enzymes Farms Insecticides Pesticides Pharmacology Phosphate esters Phosphorylation Poisoning Studies |
| title | Effects of magnesium chloride on in vitro cholinesterase and ATP ase poisoning by organophosphate (chlorpyrifos) |
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