Isolation and Characterization of Biosurfactant from Bacillus amyloliquefaciens VITANS6 Isolated from Oil Contaminated Soil collected from an Automobile Workshop in Bangalore, India

In remediation projects, it has seen that chemical surfactants sometimes form toxic compounds with the pollutants, proving to be counterproductive3. [...]despite the fact that chemical surfactants currently prove to be the cheaper option, interest has turned onto biosurfactants. Many microbes are capable of producing surface active amphoteric agents which in a growth medium helps in emulsification of hydrocarbons and also reduces the interfacial and surface tension, which in turn increase the solubility and emulsification of two immiscible phases and also provides an insoluble substrate for microorganisms, which are known as biosurfactants7. [...]with the assumption that biosurfactant producing microbes are mostly present near soils of petroleum industries, and places where oil is used extensively, organisms have been isolated from such regions and various methods have been opted for extraction for potential use. [...]the complete identification was done by using molecular characterization based on 16s rRNA sequencing. [...]the potential isolate ANS6 was identified as Bacillus amyloliquefaciens VITANS6 CONCLUSION: