Combined Hepatoprotective Activity of Murraya koenigii and Phyllanthus niruri Extracts Against Paracetamol Induced Hepatotoxicity in Alcoholic Rats
The present study elucidated the hepatoprotective activity of combination of alcohol water extracts (AWEs) of Murraya koenigii (AWEMK) and Phyllanthus niruri (AWEPN) leaves against paracetamol induced hepatic damage in ethanol exposed rats. Phytochemical analysis of AWEs determined the presence of f...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2018-06, Vol.88 (2), p.655-665 |
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| creator | Shah, Pallavi Singh, S. P. Gupta, Atul Kumar Kumar, Anil |
| description | The present study elucidated the hepatoprotective activity of combination of alcohol water extracts (AWEs) of
Murraya koenigii
(AWEMK) and
Phyllanthus niruri
(AWEPN) leaves against paracetamol induced hepatic damage in ethanol exposed rats. Phytochemical analysis of AWEs determined the presence of flavonoids and phenols, followed by high performance liquid chromatography determination of an important antioxidant tannic acid in AWEMK and a major bioactive lignan phyllanthin in AWEPN. Gas chromatography mass spectrometric analysis revealed the presence of carbazole alkaloids in AWEMK whereas AWEPN was found rich in sterols. Prolonged consumption of ethanol has been reported to enhance the hepatic damage caused by paracetamol overdose by induction of cytochrome P450 (CYP450) and depletion of reduced glutathione (GSH) in alcohol abusers. Oral administration of alcohol (5 ml/kg/day) for three days followed by paracetamol (500 mg/kg, b.i.d.) for another 3 days produced liver damage in rats as manifested by the significant (
P
|
| doi_str_mv | 10.1007/s40011-016-0800-5 |
| format | Article |
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Murraya koenigii
(AWEMK) and
Phyllanthus niruri
(AWEPN) leaves against paracetamol induced hepatic damage in ethanol exposed rats. Phytochemical analysis of AWEs determined the presence of flavonoids and phenols, followed by high performance liquid chromatography determination of an important antioxidant tannic acid in AWEMK and a major bioactive lignan phyllanthin in AWEPN. Gas chromatography mass spectrometric analysis revealed the presence of carbazole alkaloids in AWEMK whereas AWEPN was found rich in sterols. Prolonged consumption of ethanol has been reported to enhance the hepatic damage caused by paracetamol overdose by induction of cytochrome P450 (CYP450) and depletion of reduced glutathione (GSH) in alcohol abusers. Oral administration of alcohol (5 ml/kg/day) for three days followed by paracetamol (500 mg/kg, b.i.d.) for another 3 days produced liver damage in rats as manifested by the significant (
P
< 0.01) rise in levels of liver function tests and a marked increase in lipid peroxidation associated with significant reduction of the hepatic GSH and superoxide dismutase activity as compared with respective control values. Post-treatment of rats with silymarin (200 mg/kg), AWEMK (500 mg/kg), AWEPN (500 mg/kg) and combination of AWEMK and AWEPN (250 mg/kg each) for one week significantly (
P
< 0.01) inhibited these biochemical alterations which were confirmed by histopathological examination of the liver. Among all the treatment groups, the combination of extracts showed significant reversal of hepatic damage thus accentuating its hepatoprotective potential and immense value for use as a hepatoprotectant.</description><identifier>ISSN: 0369-8211</identifier><identifier>EISSN: 2250-1746</identifier><identifier>DOI: 10.1007/s40011-016-0800-5</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Alcohol ; Alkaloids ; Analgesics ; Antioxidants ; Behavioral Sciences ; Biomedical and Life Sciences ; Carbazole ; Chromatography ; Cytochrome P450 ; Ethanol ; Flavonoids ; Gas chromatography ; Glutathione ; Hepatotoxicity ; High-performance liquid chromatography ; Life Sciences ; Lipid peroxidation ; Liver ; Murraya koenigii ; Nucleic Acid Chemistry ; Oral administration ; Overdose ; Paracetamol ; Phenols ; Phyllanthus niruri ; Plant Biochemistry ; Research Article ; Rodents ; Silymarin ; Sterols ; Superoxide dismutase ; Tannic acid</subject><ispartof>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences, 2018-06, Vol.88 (2), p.655-665</ispartof><rights>The National Academy of Sciences, India 2016</rights><rights>Copyright Springer Science & Business Media 2018</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2315-8e2049b0573c45ff886462277ee8da3760363511f028dfd86cb29da8d7ae08e23</citedby><cites>FETCH-LOGICAL-c2315-8e2049b0573c45ff886462277ee8da3760363511f028dfd86cb29da8d7ae08e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40011-016-0800-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40011-016-0800-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>309,310,314,777,781,786,787,23911,23912,25121,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Shah, Pallavi</creatorcontrib><creatorcontrib>Singh, S. P.</creatorcontrib><creatorcontrib>Gupta, Atul Kumar</creatorcontrib><creatorcontrib>Kumar, Anil</creatorcontrib><title>Combined Hepatoprotective Activity of Murraya koenigii and Phyllanthus niruri Extracts Against Paracetamol Induced Hepatotoxicity in Alcoholic Rats</title><title>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</title><addtitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</addtitle><description>The present study elucidated the hepatoprotective activity of combination of alcohol water extracts (AWEs) of
Murraya koenigii
(AWEMK) and
Phyllanthus niruri
(AWEPN) leaves against paracetamol induced hepatic damage in ethanol exposed rats. Phytochemical analysis of AWEs determined the presence of flavonoids and phenols, followed by high performance liquid chromatography determination of an important antioxidant tannic acid in AWEMK and a major bioactive lignan phyllanthin in AWEPN. Gas chromatography mass spectrometric analysis revealed the presence of carbazole alkaloids in AWEMK whereas AWEPN was found rich in sterols. Prolonged consumption of ethanol has been reported to enhance the hepatic damage caused by paracetamol overdose by induction of cytochrome P450 (CYP450) and depletion of reduced glutathione (GSH) in alcohol abusers. Oral administration of alcohol (5 ml/kg/day) for three days followed by paracetamol (500 mg/kg, b.i.d.) for another 3 days produced liver damage in rats as manifested by the significant (
P
< 0.01) rise in levels of liver function tests and a marked increase in lipid peroxidation associated with significant reduction of the hepatic GSH and superoxide dismutase activity as compared with respective control values. Post-treatment of rats with silymarin (200 mg/kg), AWEMK (500 mg/kg), AWEPN (500 mg/kg) and combination of AWEMK and AWEPN (250 mg/kg each) for one week significantly (
P
< 0.01) inhibited these biochemical alterations which were confirmed by histopathological examination of the liver. Among all the treatment groups, the combination of extracts showed significant reversal of hepatic damage thus accentuating its hepatoprotective potential and immense value for use as a hepatoprotectant.</description><subject>Alcohol</subject><subject>Alkaloids</subject><subject>Analgesics</subject><subject>Antioxidants</subject><subject>Behavioral Sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Carbazole</subject><subject>Chromatography</subject><subject>Cytochrome P450</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Gas chromatography</subject><subject>Glutathione</subject><subject>Hepatotoxicity</subject><subject>High-performance liquid chromatography</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Liver</subject><subject>Murraya koenigii</subject><subject>Nucleic Acid Chemistry</subject><subject>Oral administration</subject><subject>Overdose</subject><subject>Paracetamol</subject><subject>Phenols</subject><subject>Phyllanthus niruri</subject><subject>Plant Biochemistry</subject><subject>Research Article</subject><subject>Rodents</subject><subject>Silymarin</subject><subject>Sterols</subject><subject>Superoxide dismutase</subject><subject>Tannic acid</subject><issn>0369-8211</issn><issn>2250-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1UMtKAzEUDaJgqf0AdwHXozeZmSRdllKtoCii65BmMm3qNKlJRtrv8IdNqejKuzlcOA_OQeiSwDUB4DexAiCkAMIKEABFfYIGlNZQEF6xUzSAko0LQQk5R6MY15CP1UAqMUBfU79ZWGcaPDdblfw2-GR0sp8GTw5g0x77Fj_2Iai9wu_eOLu0FivX4OfVvuuUS6s-YmdDHyye7VJQOkU8WSrrYsLPKv8mqY3v8L1rev2blPzO6oO9dXjSab_yndX4RaV4gc5a1UUz-sEherudvU7nxcPT3f108lBoWpK6EIZCNV5AzUtd1W0rBKsYpZwbIxpVcpZrlzUhLVDRtI1gekHHjRINVwayuByiq6NvLv3Rm5jk2vfB5UhJoRScVRR4ZpEjSwcfYzCt3Aa7UWEvCcjD_PI4v8zzy8P8ss4aetTEzHVLE_6c_xd9A8xSicw</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Shah, Pallavi</creator><creator>Singh, S. P.</creator><creator>Gupta, Atul Kumar</creator><creator>Kumar, Anil</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180601</creationdate><title>Combined Hepatoprotective Activity of Murraya koenigii and Phyllanthus niruri Extracts Against Paracetamol Induced Hepatotoxicity in Alcoholic Rats</title><author>Shah, Pallavi ; Singh, S. P. ; Gupta, Atul Kumar ; Kumar, Anil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2315-8e2049b0573c45ff886462277ee8da3760363511f028dfd86cb29da8d7ae08e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alcohol</topic><topic>Alkaloids</topic><topic>Analgesics</topic><topic>Antioxidants</topic><topic>Behavioral Sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Carbazole</topic><topic>Chromatography</topic><topic>Cytochrome P450</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Gas chromatography</topic><topic>Glutathione</topic><topic>Hepatotoxicity</topic><topic>High-performance liquid chromatography</topic><topic>Life Sciences</topic><topic>Lipid peroxidation</topic><topic>Liver</topic><topic>Murraya koenigii</topic><topic>Nucleic Acid Chemistry</topic><topic>Oral administration</topic><topic>Overdose</topic><topic>Paracetamol</topic><topic>Phenols</topic><topic>Phyllanthus niruri</topic><topic>Plant Biochemistry</topic><topic>Research Article</topic><topic>Rodents</topic><topic>Silymarin</topic><topic>Sterols</topic><topic>Superoxide dismutase</topic><topic>Tannic acid</topic><toplevel>online_resources</toplevel><creatorcontrib>Shah, Pallavi</creatorcontrib><creatorcontrib>Singh, S. P.</creatorcontrib><creatorcontrib>Gupta, Atul Kumar</creatorcontrib><creatorcontrib>Kumar, Anil</creatorcontrib><collection>CrossRef</collection><jtitle>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shah, Pallavi</au><au>Singh, S. P.</au><au>Gupta, Atul Kumar</au><au>Kumar, Anil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined Hepatoprotective Activity of Murraya koenigii and Phyllanthus niruri Extracts Against Paracetamol Induced Hepatotoxicity in Alcoholic Rats</atitle><jtitle>Proceedings of the National Academy of Sciences, India. Section B: Biological sciences</jtitle><stitle>Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci</stitle><date>2018-06-01</date><risdate>2018</risdate><volume>88</volume><issue>2</issue><spage>655</spage><epage>665</epage><pages>655-665</pages><issn>0369-8211</issn><eissn>2250-1746</eissn><abstract>The present study elucidated the hepatoprotective activity of combination of alcohol water extracts (AWEs) of
Murraya koenigii
(AWEMK) and
Phyllanthus niruri
(AWEPN) leaves against paracetamol induced hepatic damage in ethanol exposed rats. Phytochemical analysis of AWEs determined the presence of flavonoids and phenols, followed by high performance liquid chromatography determination of an important antioxidant tannic acid in AWEMK and a major bioactive lignan phyllanthin in AWEPN. Gas chromatography mass spectrometric analysis revealed the presence of carbazole alkaloids in AWEMK whereas AWEPN was found rich in sterols. Prolonged consumption of ethanol has been reported to enhance the hepatic damage caused by paracetamol overdose by induction of cytochrome P450 (CYP450) and depletion of reduced glutathione (GSH) in alcohol abusers. Oral administration of alcohol (5 ml/kg/day) for three days followed by paracetamol (500 mg/kg, b.i.d.) for another 3 days produced liver damage in rats as manifested by the significant (
P
< 0.01) rise in levels of liver function tests and a marked increase in lipid peroxidation associated with significant reduction of the hepatic GSH and superoxide dismutase activity as compared with respective control values. Post-treatment of rats with silymarin (200 mg/kg), AWEMK (500 mg/kg), AWEPN (500 mg/kg) and combination of AWEMK and AWEPN (250 mg/kg each) for one week significantly (
P
< 0.01) inhibited these biochemical alterations which were confirmed by histopathological examination of the liver. Among all the treatment groups, the combination of extracts showed significant reversal of hepatic damage thus accentuating its hepatoprotective potential and immense value for use as a hepatoprotectant.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s40011-016-0800-5</doi><tpages>11</tpages></addata></record> |
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| subjects | Alcohol Alkaloids Analgesics Antioxidants Behavioral Sciences Biomedical and Life Sciences Carbazole Chromatography Cytochrome P450 Ethanol Flavonoids Gas chromatography Glutathione Hepatotoxicity High-performance liquid chromatography Life Sciences Lipid peroxidation Liver Murraya koenigii Nucleic Acid Chemistry Oral administration Overdose Paracetamol Phenols Phyllanthus niruri Plant Biochemistry Research Article Rodents Silymarin Sterols Superoxide dismutase Tannic acid |
| title | Combined Hepatoprotective Activity of Murraya koenigii and Phyllanthus niruri Extracts Against Paracetamol Induced Hepatotoxicity in Alcoholic Rats |
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