Effects of Remineralization-accelerating Agent in Odontoblast-like Cell (MDPC-23)

It has been reported that RE-06 experimentally produced as a remineralization-accelerating agent by GC Corp., induces remineralization of demineralized dentin. We analyzed the effect of RE-06 on odontoblast-like cell (MDPC-23) by determining cell proliferation and gene expression related to mineralization.A new media with RE-06 liquid-A, B and a mixture were exposed into the MDPC-23 cells respectively. The cells were then incubated for 1, 2 and 4 days. Then mRNA expression of BMP-2, 4 and Cbfa1 genes were analyzed by RT-PCR. Cell proliferation was done of MDPC-23, and was measured after 1, 2 and 4 days.For the group containing liquid-A, BMP-2 and 4 were expressed only on day 2. Cbfa1 expression was seen only during day 2 and 4.For the group with mixed A and B, BMP-2 and Cbfa1 were expressed at day 1 and 2 and BMP-4 was expressed on all days. Cell proliferation containing RE-06 was lower compared to the control. No cell growth was observed in liquid-A or with mixed A and B. These findings suggest that RE-06, particularly liquid-A regulates the expression of mineralization–related genes and inhibits the growth of MDPC-23 cells.