Practical fertilization procedure and embryonic development of the New Zealand geoduck clam (Panopea zelandica)

Cultivation of the geoduck Panopea zelandica (Quoy & Gaimard, 1835) requires knowledge on embryonic development to produce spat in hatcheries. This study investigated the development of P. zelandica embryos at 15°C and 35 ppt and the optimal sperm:egg ratios for fertilization under hatchery cond...

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Veröffentlicht in:	Journal of the Marine Biological Association of the United Kingdom 2018-05, Vol.98 (3), p.475-484
Hauptverfasser:	Le, Dung V., Young, Tim, Alfaro, Andrea C., Ragg, Norman L.C., Hilton, Zoë, Watts, Ellie, King, Nick
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal embryos Aquaculture Biological fertilization Bivalvia Blastula Breeding stock Clams Crassostrea gigas Cultivation Eggs Embryogenesis Embryonic development Embryonic growth stage Embryos Fertilization Fish hatcheries Gastrulation Hatcheries Larvae Mental depression Mollusks Oysters Panopea Panopea abrupta Panopea generosa R&D Ratios Research & development Serotonin Spat Sperm Spermatozoa Studies Success Veligers
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Zusammenfassung:	Cultivation of the geoduck Panopea zelandica (Quoy & Gaimard, 1835) requires knowledge on embryonic development to produce spat in hatcheries. This study investigated the development of P. zelandica embryos at 15°C and 35 ppt and the optimal sperm:egg ratios for fertilization under hatchery conditions. Panopea zelandica broodstock were induced to spawn by serotonin injection. Sperm and eggs were collected and fertilization was conducted at sperm:egg ratios of: 50:1, 100:1, 500:1, 1000:1 and 10,000:1 over 40 min. The optimal sperm:egg ratio was
ISSN:	0025-3154 1469-7769
DOI:	10.1017/S0025315416001685