Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance
Objective To investigate the expression of SFRP1 gene methylation and SFRP1 protein in lung adenocarcinoma tissues. Methods There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP...
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| Veröffentlicht in: | Zhongliu fangzhi yanjiu 2017-05, Vol.44 (5), p.320 |
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| creator | Li, Xiaojie Yin, Guilin Dong, Yongqiang Zhu, Shuibo Zhang, Xiaoming Ji, Tao Xu, Jiahang |
| description | Objective To investigate the expression of SFRP1 gene methylation and SFRP1 protein in lung adenocarcinoma tissues. Methods There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP1 protein in lung adenocarcinoma tissues and adjacent tissues were detected respectively by methylation specific PCR, real-time PCR and immunohistochemistry. Results SFRP1 gene methylation rate was higher in tumor tissues than that in adjacent tissues (58.3% vs. 20.0%, P=0.001). The relative expression of SFRP1 mRNA in tumor tissues and adjacent tissues were (0.847±0.297) and (2.019±0.445) (P |
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Methods There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP1 protein in lung adenocarcinoma tissues and adjacent tissues were detected respectively by methylation specific PCR, real-time PCR and immunohistochemistry. Results SFRP1 gene methylation rate was higher in tumor tissues than that in adjacent tissues (58.3% vs. 20.0%, P=0.001). The relative expression of SFRP1 mRNA in tumor tissues and adjacent tissues were (0.847±0.297) and (2.019±0.445) (P<0.01). The positive expression rate of SFRP1 protein in tumor tissues was significantly lower than that in adjacent tissues (38.3%(23/60) vs. 83.3%(25/30), (P<0.001)). SFRP1 protein expression was correlated with differentiation degree, clinical stage and lymph node metastasis (P<0.05). SFRP1 gene methylation was closely corre</description><identifier>ISSN: 1000-8578</identifier><language>chi</language><publisher>Tianjin: China Anti-Cancer Association</publisher><subject>Adenocarcinoma ; Clinical significance ; Differentiation ; DNA methylation ; Frizzled-related protein 1 ; Gene expression ; Immunohistochemistry ; Lung cancer ; Lungs ; Lymph nodes ; Lymphatic system ; Metastases ; Metastasis ; mRNA ; Protein deficiency ; Protein expression ; Proteins ; Surgery ; Tissues ; Tumors</subject><ispartof>Zhongliu fangzhi yanjiu, 2017-05, Vol.44 (5), p.320</ispartof><rights>Copyright China Anti-Cancer Association May 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Li, Xiaojie</creatorcontrib><creatorcontrib>Yin, Guilin</creatorcontrib><creatorcontrib>Dong, Yongqiang</creatorcontrib><creatorcontrib>Zhu, Shuibo</creatorcontrib><creatorcontrib>Zhang, Xiaoming</creatorcontrib><creatorcontrib>Ji, Tao</creatorcontrib><creatorcontrib>Xu, Jiahang</creatorcontrib><title>Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance</title><title>Zhongliu fangzhi yanjiu</title><description>Objective To investigate the expression of SFRP1 gene methylation and SFRP1 protein in lung adenocarcinoma tissues. Methods There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP1 protein in lung adenocarcinoma tissues and adjacent tissues were detected respectively by methylation specific PCR, real-time PCR and immunohistochemistry. Results SFRP1 gene methylation rate was higher in tumor tissues than that in adjacent tissues (58.3% vs. 20.0%, P=0.001). The relative expression of SFRP1 mRNA in tumor tissues and adjacent tissues were (0.847±0.297) and (2.019±0.445) (P<0.01). The positive expression rate of SFRP1 protein in tumor tissues was significantly lower than that in adjacent tissues (38.3%(23/60) vs. 83.3%(25/30), (P<0.001)). SFRP1 protein expression was correlated with differentiation degree, clinical stage and lymph node metastasis (P<0.05). SFRP1 gene methylation was closely corre</description><subject>Adenocarcinoma</subject><subject>Clinical significance</subject><subject>Differentiation</subject><subject>DNA methylation</subject><subject>Frizzled-related protein 1</subject><subject>Gene expression</subject><subject>Immunohistochemistry</subject><subject>Lung cancer</subject><subject>Lungs</subject><subject>Lymph nodes</subject><subject>Lymphatic system</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>mRNA</subject><subject>Protein deficiency</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Surgery</subject><subject>Tissues</subject><subject>Tumors</subject><issn>1000-8578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqNi80KwjAQhHNQUNR3WPAsbFq1vUrx56BQ1LvEdlsjcVOTFvTtDeIDCAMzzDfTE0OJiLN0kaQDMfFeXxGlxHmc4FA061fjKJSWPdgKTptjLmFLTHCg9vY2qg0IFJc_lDvbkmYI2ndcw6oktoVyhWb7UN_hkcKLSsiMZl0oAydds65C5ILGol8p42ny85GYbtbnbDdrnH125NvL3XaOA7pEGCUYLzFN4v9WHwGPSWw</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Li, Xiaojie</creator><creator>Yin, Guilin</creator><creator>Dong, Yongqiang</creator><creator>Zhu, Shuibo</creator><creator>Zhang, Xiaoming</creator><creator>Ji, Tao</creator><creator>Xu, Jiahang</creator><general>China Anti-Cancer Association</general><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20170501</creationdate><title>Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance</title><author>Li, Xiaojie ; Yin, Guilin ; Dong, Yongqiang ; Zhu, Shuibo ; Zhang, Xiaoming ; Ji, Tao ; Xu, Jiahang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_20270360873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2017</creationdate><topic>Adenocarcinoma</topic><topic>Clinical significance</topic><topic>Differentiation</topic><topic>DNA methylation</topic><topic>Frizzled-related protein 1</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>Lung cancer</topic><topic>Lungs</topic><topic>Lymph nodes</topic><topic>Lymphatic system</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>mRNA</topic><topic>Protein deficiency</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Surgery</topic><topic>Tissues</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaojie</creatorcontrib><creatorcontrib>Yin, Guilin</creatorcontrib><creatorcontrib>Dong, Yongqiang</creatorcontrib><creatorcontrib>Zhu, Shuibo</creatorcontrib><creatorcontrib>Zhang, Xiaoming</creatorcontrib><creatorcontrib>Ji, Tao</creatorcontrib><creatorcontrib>Xu, Jiahang</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Zhongliu fangzhi yanjiu</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaojie</au><au>Yin, Guilin</au><au>Dong, Yongqiang</au><au>Zhu, Shuibo</au><au>Zhang, Xiaoming</au><au>Ji, Tao</au><au>Xu, Jiahang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance</atitle><jtitle>Zhongliu fangzhi yanjiu</jtitle><date>2017-05-01</date><risdate>2017</risdate><volume>44</volume><issue>5</issue><spage>320</spage><pages>320-</pages><issn>1000-8578</issn><abstract>Objective To investigate the expression of SFRP1 gene methylation and SFRP1 protein in lung adenocarcinoma tissues. Methods There were 60 lung adenocarcinoma patients who underwent a completely surgical excision from May 2015 to May 2016. The expression of SFRP1 gene methylation, SFRP1 mRNA and SFRP1 protein in lung adenocarcinoma tissues and adjacent tissues were detected respectively by methylation specific PCR, real-time PCR and immunohistochemistry. Results SFRP1 gene methylation rate was higher in tumor tissues than that in adjacent tissues (58.3% vs. 20.0%, P=0.001). The relative expression of SFRP1 mRNA in tumor tissues and adjacent tissues were (0.847±0.297) and (2.019±0.445) (P<0.01). The positive expression rate of SFRP1 protein in tumor tissues was significantly lower than that in adjacent tissues (38.3%(23/60) vs. 83.3%(25/30), (P<0.001)). SFRP1 protein expression was correlated with differentiation degree, clinical stage and lymph node metastasis (P<0.05). SFRP1 gene methylation was closely corre</abstract><cop>Tianjin</cop><pub>China Anti-Cancer Association</pub></addata></record> |
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| language | chi |
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| source | Directory of Open Access Journals |
| subjects | Adenocarcinoma Clinical significance Differentiation DNA methylation Frizzled-related protein 1 Gene expression Immunohistochemistry Lung cancer Lungs Lymph nodes Lymphatic system Metastases Metastasis mRNA Protein deficiency Protein expression Proteins Surgery Tissues Tumors |
| title | Expressions of SFRP1 Gene Methylation and SFRP1 Protein in Lung Adenocarcinoma and Related Clinical Significance |
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