A putative two pore channel AtTPC1 mediates Ca2+ flux in arabidopsis leaf cells

The gene encoding voltage-gated channel with high affinity for Ca**2+ permeation has not been cloned from plants. In the present study, we isolated a full-length CDNA encoding a putative Ca2+ channel (AtTPC1) from Arabidopsis. AtTPC1 has two conserved homologous domains, both of which contain six transmembrane segments (S1-S6) and a pore loop (P) between S5 and S6 in each domain, and has the highest homology with the two pore channel TPC1 recently cloned from rat. The overall structure is similar to the half of the general structure of alpha-subunits of voltageactivated Ca**2+ channels from animals. AtTPC1 rescued the Ca**2+ uptake activity of a yeast mutant cchl. Sucroseinduced luminescence, which reflects a cytosolic free Ca**2+ increase in aequorin-expressing Arabidopsis leaves, was enhanced by overexpression of AtTPC1 and suppressed by antisense expression of it. Sucrose-H+ symporters AtSUC1 and 2, which depolarize membrane potential of cells receiving sucrose, also depressed a Ca2+ increase by their antisense expression. These results suggest that AtTPC1 mediates a voltage-activated Ca**2+ influx in Arabidopsis leaf cells.